Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613165402_7tn5 AACAAAAAGAAGAGCGACGCCATCTCTTTTAG(+AGGACA)GTACCTGTTTCTGGATTAAGCTGGATTCTGC NGG 10 8 17 8 24 0 100 5 10
    240613165400_gu37 TATTCCGGTTGAGGAGTAGACAACTGACTGGG(+AGGACA)GATCCAAACCAATTTTTACATGGCCTTGAGG NGG 10 8 17 8 24 0 100 5 10
    240613165359_xdxh TCACCCCTCAAGGCCATGTAAAAATTGGTTTG(+AGGACA)GATCCCCAGTCAGTTGTCTACTCCTCAACCG NGG 10 8 17 8 24 0 100 5 10
    240613165357_ul4b AGTCTTAGCTTGCTTCCTGGTATTCGTGGTAG(+AGGACA)GATCCTGTCACAGGTCAATGTTAAGACTCAA NGG 10 8 17 8 24 0 100 5 10
    240613165356_g13o AAATTTTGAGTCTTAACATTGACCTGTGACAG(+AGGACA)GATCCTACCACGAATACCAGGAAGCAAGCTA NGG 10 8 17 8 24 0 100 5 10
    240613165353_n6uw ACAGGTCAACAACTTTCTTTCTTGGTTTCCTG(+AGGACA)GTACCCTAGCCCCCCTCACACCCGTCTCACC NGG 10 8 17 8 24 0 100 5 10
    240613165351_vgmy GCAAAGGTGAGACGGGTGTGAGGGGGGCTAGG(+AGGACA)GTACCAGGAAACCAAGAAAGAAAGTTGTTGA NGG 10 8 17 8 24 0 100 5 10
    240613165349_dxwn CATCTGAGTTCATGCATCGAGCCTGGGCCTGG(+AGGACA)GATCCAGTGAAGCTAAGCCCTCCTCCACCCA NGG 10 8 17 8 24 0 100 5 10
    240613165348_8d1g GGCCCTGGGTGGAGGAGGGCTTAGCTTCACTG(+AGGACA)GATCCCAGGCCCAGGCTCGATGCATGAACTC NGG 10 8 17 8 24 0 100 5 10
    240613165347_p3x9 CCCTGAGGGATCCTGGCCCCTGCTGAGGACTG(+AGGACA)GTACCTATTTCTCATTAAAGTGGCTTTGATC NGG 10 8 17 8 24 0 100 5 10