Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613165549_40vr TTCCCAGTTAGTTAGGGGTATGCTCCAAGAAG(+AGGACA)GATCCTAGAGGTCCTGTGGGGATGTTGAATT NGG 10 8 17 8 24 0 100 5 10
    240613165548_9tif ACTACAATTCAACATCCCCACAGGACCTCTAG(+AGGACA)GATCCTTCTTGGAGCATACCCCTAACTAACT NGG 10 8 17 8 24 0 100 5 10
    240613165546_nxty GAAGTGTGCCCAAGGTTAGAGAGTACAGAGTG(+AGGACA)GATCCCCCTCCCCCCCAAAAAAGATAGTAAT NGG 10 8 17 8 24 0 100 5 10
    240613165544_20wl AGAACATTACTATCTTTTTTGGGGGGGAGGGG(+AGGACA)GATCCACTCTGTACTCTCTAACCTTGGGCAC NGG 10 8 17 8 24 0 100 5 10
    240613165543_9i6q CTTTGTGGATACCTAGAAGGAGGTCTTGAGAG(+AGGACA)GATCCAGTATTAATGGTGTCATAGAGACCAG NGG 10 8 17 8 24 0 100 5 10
    240613165541_5y7m GCTCTCTGGTCTCTATGACACCATTAATACTG(+AGGACA)GATCCTCTCAAGACCTCCTTCTAGGTATCCA NGG 10 8 17 8 24 0 100 5 10
    240613165540_ajy8 TGGCCACAAGTAACAAGAGTGGCAAGAATCTG(+AGGACA)GATCCCCAAGCTTCAGCCAACCAACAGGTTA NGG 10 8 17 8 24 0 100 5 10
    240613165538_vwzm TCCATTAACCTGTTGGTTGGCTGAAGCTTGGG(+AGGACA)GATCCAGATTCTTGCCACTCTTGTTACTTGT NGG 10 8 17 8 24 0 100 5 10
    240613165537_yd1x GGTAATTGAGTTTTGAATCTTATTAACTTATG(+AGGACA)GATCCATCATACTTTCAAGTTTCCATAACAT NGG 10 8 17 8 24 0 100 5 10
    240613165535_vcpg AATGTATGTTATGGAAACTTGAAAGTATGATG(+AGGACA)GATCCATAAGTTAATAAGATTCAAAACTCAA NGG 10 8 17 8 24 0 100 5 10