Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613165617_9w8h TCATAAATCTCTGTATTGGGAAAAGACTTAGG(+AGGACA)GTACCCAAGAAACAGAAAATTTAGCTGAGAA NGG 10 8 17 8 24 0 100 5 10
    240613165615_316q GCCCATTCTCAGCTAAATTTTCTGTTTCTTGG(+AGGACA)GTACCCTAAGTCTTTTCCCAATACAGAGATT NGG 10 8 17 8 24 0 100 5 10
    240613165614_fyjh CTGAAAATTTGCATCCTATCAGGGACCATGAG(+AGGACA)GTACCCTAGGAAGGATTTTTGCATACTGATT NGG 10 8 17 8 24 0 100 5 10
    240613165612_hwc7 CAAGTAATCAGTATGCAAAAATCCTTCCTAGG(+AGGACA)GTACCTCATGGTCCCTGATAGGATGCAAATT NGG 10 8 17 8 24 0 100 5 10
    240613165610_onl2 TGCTAAAGGAAATGTACTCAGCTTATAAAATG(+AGGACA)GTACCAGCTTTGCTATATTTATCGTAGCAAT NGG 10 8 17 8 24 0 100 5 10
    240613165609_66gs TGAACATTGCTACGATAAATATAGCAAAGCTG(+AGGACA)GTACCATTTTATAAGCTGAGTACATTTCCTT NGG 10 8 17 8 24 0 100 5 10
    240613165608_7ois TTGAAAAATAAAATTAATAATTTAATTGGATG(+AGGACA)GTACCAGTAACTAAACCGACTCCTGTTCATT NGG 10 8 17 8 24 0 100 5 10
    240613165606_gzsp CCTTCAATGAACAGGAGTCGGTTTAGTTACTG(+AGGACA)GTACCATCCAATTAAATTATTAATTTTATTT NGG 10 8 17 8 24 0 100 5 10
    240613165605_bd36 AGTGTGAAATAAACAGGATAAAAAAGAAAATG(+AGGACA)GTACCATGAGCAGGCAGAATGCTGCAATAAT NGG 10 8 17 8 24 0 100 5 10
    240613165604_0qz9 TATCAATTATTGCAGCATTCTGCCTGCTCATG(+AGGACA)GTACCATTTTCTTTTTTATCCTGTTTATTTC NGG 10 8 17 8 24 0 100 5 10