Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613165837_e8hr GTAGTGAGATGTAGTATTTACTCTTAACATAG(+AGGACA)GATCCTGTGGAACAGGTGTTCTGAGAAGACT NGG 10 8 17 8 24 0 100 5 10
    240613165835_gh3y AATTCAGTCTTCTCAGAACACCTGTTCCACAG(+AGGACA)GATCCTATGTTAAGAGTAAATACTACATCTC NGG 10 8 17 8 24 0 100 5 10
    240613165834_ntji TTAGTCTGTAGCTAATTTCTAAGAAAAACAAG(+AGGACA)GTACCTTCAATTAAAATTAGAGCTTAGAATA NGG 10 8 17 8 24 0 100 5 10
    240613165832_uwp6 TTTTATATTCTAAGCTCTAATTTTAATTGAAG(+AGGACA)GTACCTTGTTTTTCTTAGAAATTAGCTACAG NGG 10 8 17 8 24 0 100 5 10
    240613165831_s0kf TTAACTACAGAACGTAGAAAGCAGTGGAGTGG(+AGGACA)GTACCGGACCTGTCGCCACACACCCTTACCT NGG 10 8 17 8 24 0 100 5 10
    240613165829_jhfz TTCTTAGGTAAGGGTGTGTGGCGACAGGTCCG(+AGGACA)GTACCCACTCCACTGCTTTCTACGTTCTGTA NGG 10 8 17 8 24 0 100 5 10
    240613165827_nibi ATGTGTGTACAACCTTGAGCATCATTCCTTAG(+AGGACA)GATCCCCGCACATGGGCCCCACATCTCTAGA NGG 10 8 17 8 24 0 100 5 10
    240613165825_by11 GGCACTCTAGAGATGTGGGGCCCATGTGCGGG(+AGGACA)GATCCTAAGGAATGATGCTCAAGGTTGTACA NGG 10 8 17 8 24 0 100 5 10
    240613165824_5uzo AGACAGGTTGCTGAGGCTGGCTTTGAACTTGG(+AGGACA)GATCCTGAGGCTTCAGACTCCCAGGTGGCTG NGG 10 8 17 8 24 0 100 5 10
    240613165822_vdub ATAAACAGCCACCTGGGAGTCTGAAGCCTCAG(+AGGACA)GATCCCAAGTTCAAAGCCAGCCTCAGCAACC NGG 10 8 17 8 24 0 100 5 10