Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613165853_ll6d CCTCCTGAGAGCCAGGATATAGTTTACTGGGG(+AGGACA)GATCCGTGAAGAATTCGGTTCCTGTCCTGAC NGG 10 8 17 8 24 0 100 5 10
    240613165852_9zl7 GCTAGGTCAGGACAGGAACCGAATTCTTCACG(+AGGACA)GATCCCCCAGTAAACTATATCCTGGCTCTCA NGG 10 8 17 8 24 0 100 5 10
    240613165850_ndql TGACTGAGTGGGCACTTAATAATGAATGTTTG(+AGGACA)GATCCTAAGCTCTGATGTTCATTGCTCTCTA NGG 10 8 17 8 24 0 100 5 10
    240613165849_54je GAAAATAGAGAGCAATGAACATCAGAGCTTAG(+AGGACA)GATCCAAACATTCATTATTAAGTGCCCACTC NGG 10 8 17 8 24 0 100 5 10
    240613165848_m2go CACCCTAGTTCCAGCTCTGATTGGGTGGTCAG(+AGGACA)GATCCAGGGAAGGGCAGCATTTGATCGTATC NGG 10 8 17 8 24 0 100 5 10
    240613165845_p3w1 GGACAGATACGATCAAATGCTGCCCTTCCCTG(+AGGACA)GATCCTGACCACCCAATCAGAGCTGGAACTA NGG 10 8 17 8 24 0 100 5 10
    240613165844_317w TCTTTGAGCTAATGGTGATGTGGTATGCAGAG(+AGGACA)GTACCAGGGGCCATGGGGGTGTGTGTGCTTC NGG 10 8 17 8 24 0 100 5 10
    240613165841_9bwc GACAGGAAGCACACACACCCCCATGGCCCCTG(+AGGACA)GTACCTCTGCATACCACATCACCATTAGCTC NGG 10 8 17 8 24 0 100 5 10
    240613165839_nitw ACCCTAGAAGGTAGGGACAAGTGAAGGACTGG(+AGGACA)GATCCTTTTTGCAGTATGTTCCTTGACTCGC NGG 10 8 17 8 24 0 100 5 10
    240613165838_huys ATGTGGCGAGTCAAGGAACATACTGCAAAAAG(+AGGACA)GATCCCAGTCCTTCACTTGTCCCTACCTTCT NGG 10 8 17 8 24 0 100 5 10