Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613170000_syqj TACTGGCAACGTGTAAATAGTGTTGGTACACG(+AGGACA)GATCCAGGCTGTACTGTAAGCACAAATTTTG NGG 10 8 17 8 24 0 100 5 10
    240613165958_wcsx ATGTTCAAAATTTGTGCTTACAGTACAGCCTG(+AGGACA)GATCCGTGTACCAACACTATTTACACGTTGC NGG 10 8 17 8 24 0 100 5 10
    240613165955_475n AGGTCAGAGACCAATCTGTCATGCAACCCTCG(+AGGACA)GATCCATGTATCCCATAAACTCCCCCTTATC NGG 10 8 17 8 24 0 100 5 10
    240613165954_9xcp GGGCTGATAAGGGGGAGTTTATGGGATACATG(+AGGACA)GATCCGAGGGTTGCATGACAGATTGGTCTCT NGG 10 8 17 8 24 0 100 5 10
    240613165952_951e TGTTACTACATCTGGCTGCAGTAAGACTTTTG(+AGGACA)GATCCAATGTAAACAGGAAGGGTAAGCATGT NGG 10 8 17 8 24 0 100 5 10
    240613165951_zgmh GGGGAACATGCTTACCCTTCCTGTTTACATTG(+AGGACA)GATCCAAAAGTCTTACTGCAGCCAGATGTAG NGG 10 8 17 8 24 0 100 5 10
    240613165950_mkxt TGTATGTATGTGTGCATATCATGTGTGTTCTG(+AGGACA)GTACCTGTGGAGATTAGGAGAGACTGTCCGG NGG 10 8 17 8 24 0 100 5 10
    240613165948_0dag GGGCACCGGACAGTCTCTCCTAATCTCCACAG(+AGGACA)GTACCAGAACACACATGATATGCACACATAC NGG 10 8 17 8 24 0 100 5 10
    240613165947_ad1c AGAGGCCGGGTTTACTTTGATAGGCTAAATAG(+AGGACA)GTACCCCAGTTAGCTGTTTGTTTTGGATTTG NGG 10 8 17 8 24 0 100 5 10
    240613165945_92m0 ATTCTCAAATCCAAAACAAACAGCTAACTGGG(+AGGACA)GTACCTATTTAGCCTATCAAAGTAAACCCGG NGG 10 8 17 8 24 0 100 5 10