Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613170104_cmkt TCTATGTGTATGTGTATATCTGCAGCATGTAG(+AGGACA)GTACCCAAAGTCTAAAGAGTGCATCAGATCA NGG 10 8 17 8 24 0 100 5 10
    240613170102_77kl CTTGGTGATCTGATGCACTCTTTAGACTTTGG(+AGGACA)GTACCTACATGCTGCAGATATACACATACAC NGG 10 8 17 8 24 0 100 5 10
    240613170100_8j5n GTGGTGAGCACATGGCGCGGGGGGCGGGGGGG(+AGGACA)GATCCCATCTATAAATACAAGAAAATTAAAA NGG 10 8 17 8 24 0 100 5 10
    240613170058_jxq0 TTATTTTTTAATTTTCTTGTATTTATAGATGG(+AGGACA)GATCCCCCCCCGCCCCCCGCGCCATGTGCTC NGG 10 8 17 8 24 0 100 5 10
    240613170057_kult TCATTTTGGACATATCTTTGGAGAAATGATTG(+AGGACA)GATCCAACATTTTATAAACCAGGTAAAATTT NGG 10 8 17 8 24 0 100 5 10
    240613170056_jomn ACCAGAAATTTTACCTGGTTTATAAAATGTTG(+AGGACA)GATCCAATCATTTCTCCAAAGATATGTCCAA NGG 10 8 17 8 24 0 100 5 10
    240613170055_qosg GATCTTGAGTTTTGCACCAAGAGGGAGGAGTG(+AGGACA)GATCCAAAAAAAAATACACAAACAAACAAAC NGG 10 8 17 8 24 0 100 5 10
    240613170053_98en TGTTTGTTTGTTTGTTTGTGTATTTTTTTTTG(+AGGACA)GATCCACTCCTCCCTCTTGGTGCAAAACTCA NGG 10 8 17 8 24 0 100 5 10
    240613170052_i4qm CCTGTTCTGAATATTTCATACAGACGTGACTG(+AGGACA)GATCCTTTTAACTGGCCAGTGATTTCAAGGA NGG 10 8 17 8 24 0 100 5 10
    240613170051_2qa8 GAATATCCTTGAAATCACTGGCCAGTTAAAAG(+AGGACA)GATCCAGTCACGTCTGTATGAAATATTCAGA NGG 10 8 17 8 24 0 100 5 10