Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613170031_daf2 AACCACATGGTGGTAACAACCATCTGTAATGG(+AGGACA)GATCCGTTGCCCTCTTCTGGCGTGTCTAAAG NGG 10 8 17 8 24 0 100 5 10
    240613170030_zjgk GCGGTCTTTAGACACGCCAGAAGAGGGCAACG(+AGGACA)GATCCCATTACAGATGGTTGTTACCACCATG NGG 10 8 17 8 24 0 100 5 10
    240613170029_ri0r AACCTGAATTTCATCCTCGGGACTCAATAGTG(+AGGACA)GATCCAGAGAACCAACTTCTAAAACTTGTCC NGG 10 8 17 8 24 0 100 5 10
    240613170028_53fo AGTCAGGACAAGTTTTAGAAGTTGGTTCTCTG(+AGGACA)GATCCACTATTGAGTCCCGAGGATGAAATTC NGG 10 8 17 8 24 0 100 5 10
    240613170026_5qzb GTGTGTGCATTTACCCACAGAACAAGGCATTG(+AGGACA)GATCCCTTGGCACTGGTGTCTCTGTCTCTCT NGG 10 8 17 8 24 0 100 5 10
    240613170024_qj46 GAGACAGAGAGACAGAGACACCAGTGCCAAGG(+AGGACA)GATCCAATGCCTTGTTCTGTGGGTAAATGCA NGG 10 8 17 8 24 0 100 5 10
    240613170022_3l45 TGTTCTCGGCATTAGTTTTCAAAATGTCCATG(+AGGACA)GATCCCTCGTCATCTGAGGAGCTGATTGTAG NGG 10 8 17 8 24 0 100 5 10
    240613170020_ztou CCCTCCTACAATCAGCTCCTCAGATGACGAGG(+AGGACA)GATCCATGGACATTTTGAAAACTAATGCCGA NGG 10 8 17 8 24 0 100 5 10
    240613170017_tjax TATGAAAATGGAGGGTAGACAACAGGTATCAG(+AGGACA)GTACCCACAAAATGAACCTCACCATGAAGTA NGG 10 8 17 8 24 0 100 5 10
    240613170016_pgci TGTGGTACTTCATGGTGAGGTTCATTTTGTGG(+AGGACA)GTACCTGATACCTGTTGTCTACCCTCCATTT NGG 10 8 17 8 24 0 100 5 10