Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605173206_b2gh TCTTGGACAGGTGGGCAGAAGGGATACAATGG(+CAGTGT)GTACCAGCAAATGCTGGCTGTACCGAGGAGG NGG 10 8 17 8 24 0 100 5 10
    240605173204_590t ACTGAACCTGGAGCTGCCCAGCAGGCTTCCGG(+CAGTGT)GATCCTCCAGGGTCTGCTTCCACAGAGCCAG NGG 10 8 17 8 24 0 100 5 10
    240605173203_a0yd GACTCCTGGCTCTGTGGAAGCAGACCCTGGAG(+CAGTGT)GATCCCGGAAGCCTGCTGGGCAGCTCCAGGT NGG 10 8 17 8 24 0 100 5 10
    240605173201_63q1 GAGTCAGTTCTTGAGCCCAGCTTTCCAGTGAG(+CAGTGT)GATCCGCACCTGCGGGATGTGTGTCCTATTC NGG 10 8 17 8 24 0 100 5 10
    240605173200_v7g0 CACATGAATAGGACACACATCCCGCAGGTGCG(+CAGTGT)GATCCTCACTGGAAAGCTGGGCTCAAGAACT NGG 10 8 17 8 24 0 100 5 10
    240605173158_g9qp AATAATGCTCACTTCCCAGTCTGTGGCCACGG(+CAGTGT)GATCCCATGTGTCTTTTTGCTTGTGGCAGAT NGG 10 8 17 8 24 0 100 5 10
    240605173156_ofc2 AAGAAATCTGCCACAAGCAAAAAGACACATGG(+CAGTGT)GATCCCGTGGCCACAGACTGGGAAGTGAGCA NGG 10 8 17 8 24 0 100 5 10
    240605173155_pbsy AGGGCAGGACCTGGTCCATCTCTGCGGGACTG(+CAGTGT)GTACCAACCAATAAGGCAGAGGGGGTTGAGC NGG 10 8 17 8 24 0 100 5 10
    240605173154_6g1f GGCGAGCTCAACCCCCTCTGCCTTATTGGTTG(+CAGTGT)GTACCAGTCCCGCAGAGATGGACCAGGTCCT NGG 10 8 17 8 24 0 100 5 10
    240605173153_gv1p GGTGTCAGCAGTTGGAGCAGCCTGGAGTTCTG(+CAGTGT)GATCCTGGCTCCTAGGTGGTTTTCACATCAG NGG 10 8 17 8 24 0 100 5 10