Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605173151_cygh AGAGGCTGATGTGAAAACCACCTAGGAGCCAG(+CAGTGT)GATCCAGAACTCCAGGCTGCTCCAACTGCTG NGG 10 8 17 8 24 0 100 5 10
    240605173149_6zat TGTGTATGCAAAAACTCCTGTCCACATGGTGG(+CAGTGT)GTACCCCACGGAAGACAGCTTTCTTATTTGA NGG 10 8 17 8 24 0 100 5 10
    240605173147_zpmi GTTTTTCAAATAAGAAAGCTGTCTTCCGTGGG(+CAGTGT)GTACCCACCATGTGGACAGGAGTTTTTGCAT NGG 10 8 17 8 24 0 100 5 10
    240605173146_dlsl CACACCACATCATTCAGTCAGACTGGTTTTTG(+CAGTGT)GTACCTAAGTATTTGTGTTCAAGTAACCTTT NGG 10 8 17 8 24 0 100 5 10
    240605173145_8ju9 GCAGTAAAGGTTACTTGAACACAAATACTTAG(+CAGTGT)GTACCAAAAACCAGTCTGACTGAATGATGTG NGG 10 8 17 8 24 0 100 5 10
    240605173143_98km GGGACTTTGACAGATTGTCTTGTAGCAGGGAG(+CAGTGT)GATCCAGCCCAGCTATGGGCCTGGGATGCCA NGG 10 8 17 8 24 0 100 5 10
    240605173141_qcsk CTCCCTGGCATCCCAGGCCCATAGCTGGGCTG(+CAGTGT)GATCCTCCCTGCTACAAGACAATCTGTCAAA NGG 10 8 17 8 24 0 100 5 10
    240605173139_j5xr GAGACCCACCTGTCACCGTGTGTCATCAAGGG(+CAGTGT)GATCCGGTGGGGAAAAGAAGGGGAAACACAG NGG 10 8 17 8 24 0 100 5 10
    240605173137_uldx CTCACCTGTGTTTCCCCTTCTTTTCCCCACCG(+CAGTGT)GATCCCCTTGATGACACACGGTGACAGGTGG NGG 10 8 17 8 24 0 100 5 10
    240605173136_3u2x GCTGAGTGAGGCCACTCCCATTGAAGAGTGAG(+CAGTGT)GATCCTGGAGCCCCAAAGGCCTTTGGGAATG NGG 10 8 17 8 24 0 100 5 10