Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605173134_zbfv TAGCTCATTCCCAAAGGCCTTTGGGGCTCCAG(+CAGTGT)GATCCTCACTCTTCAATGGGAGTGGCCTCAC NGG 10 8 17 8 24 0 100 5 10
    240605173133_02sr TCCCGTCATGTTTCTCCTTACTCATCCAATTG(+CAGTGT)GTACCCACAACTGAAGAGCTCGTGTATTTGG NGG 10 8 17 8 24 0 100 5 10
    240605173131_ovyo GCCCGCCAAATACACGAGCTCTTCAGTTGTGG(+CAGTGT)GTACCAATTGGATGAGTAAGGAGAAACATGA NGG 10 8 17 8 24 0 100 5 10
    240605173130_h3pi CTCTTTTGTCTGTGGAGTCTGCTGTGTGGCAG(+CAGTGT)GATCCCAGCCCTAGAGCTGCAGGGCTGCCCT NGG 10 8 17 8 24 0 100 5 10
    240605173128_cfb3 CCCACAGGGCAGCCCTGCAGCTCTAGGGCTGG(+CAGTGT)GATCCTGCCACACAGCAGACTCCACAGACAA NGG 10 8 17 8 24 0 100 5 10
    240605173126_lkee CTGGGTGGTGGTCTGTCTGGTTCCAGTGGGAG(+CAGTGT)GTACCATCTCTTCTCCTTTGAGAAATGCCCC NGG 10 8 17 8 24 0 100 5 10
    240605173125_em1a CTGCAGGGGCATTTCTCAAAGGAGAAGAGATG(+CAGTGT)GTACCTCCCACTGGAACCAGACAGACCACCA NGG 10 8 17 8 24 0 100 5 10
    240605173124_j9x0 TCTGGGGTAGCTTACCCAGTGCTAGGCACTGG(+CAGTGT)GATCCTGAAAGTACCTTTCTTGGTGATCTGG NGG 10 8 17 8 24 0 100 5 10
    240605173122_o801 CTGTTCCAGATCACCAAGAAAGGTACTTTCAG(+CAGTGT)GATCCCAGTGCCTAGCACTGGGTAAGCTACC NGG 10 8 17 8 24 0 100 5 10
    240605173121_tgka GAAGGCAGGTCCCCAGTTGTTGGGCCAGCAGG(+CAGTGT)GTACCTTCCTTCTTCTGCAGACTGCGATAGT NGG 10 8 17 8 24 0 100 5 10