Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605173032_a06l GGGGTACGGTTGGGCCATTCACAGAGCCTGGG(+CAGTGT)GATCCAACCTCGCTGATCACTAGGAAGAGAT NGG 10 8 17 8 24 0 100 5 10
    240605173030_0ccc CAGAAGTAAAAGCAGGCCCAAGAGCAAAGGAG(+CAGTGT)GTACCAGGAGGCCCCCTGCTGGTGAGCTTGA NGG 10 8 17 8 24 0 100 5 10
    240605173028_0rqf ATGACTCAAGCTCACCAGCAGGGGGCCTCCTG(+CAGTGT)GTACCTCCTTTGCTCTTGGGCCTGCTTTTAC NGG 10 8 17 8 24 0 100 5 10
    240605173027_97et CACCAGTGTTCGGGTGGAAGCATAGGAAACAG(+CAGTGT)GATCCCGCAGTGGCAAGGTCAGGCCACCTGC NGG 10 8 17 8 24 0 100 5 10
    240605173026_fjqa CCCAGGCAGGTGGCCTGACCTTGCCACTGCGG(+CAGTGT)GATCCTGTTTCCTATGCTTCCACCCGAACAC NGG 10 8 17 8 24 0 100 5 10
    240605173024_eqig TCCAAAATGTGAATAACTTGTCAGAGCAGCTG(+CAGTGT)GTACCCCTGATCGGCTGGGTCTGCTTCCGGT NGG 10 8 17 8 24 0 100 5 10
    240605173022_jdt8 TAGCAACCGGAAGCAGACCCAGCCGATCAGGG(+CAGTGT)GTACCAGCTGCTCTGACAAGTTATTCACATT NGG 10 8 17 8 24 0 100 5 10
    240605173021_sbqu ACGAGGTGGCTGGAGGGTGCTCTCAGCTGCAG(+CAGTGT)GTACCAGACCCAGGACAGGAAATGTCAGCCA NGG 10 8 17 8 24 0 100 5 10
    240605173020_r94j GCTGATGGCTGACATTTCCTGTCCTGGGTCTG(+CAGTGT)GTACCTGCAGCTGAGAGCACCCTCCAGCCAC NGG 10 8 17 8 24 0 100 5 10
    240605173018_5jx2 GCCTCTCCAGCCCCCGCTGGGCTGCAGCCAGG(+CAGTGT)GTACCATCAACAGCCATGTTAAGGTTCTGAT NGG 10 8 17 8 24 0 100 5 10