Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605173000_t3j7 TTAAGCCCCACAGAAATATCTTACACATTGAG(+CAGTGT)GTACCTCAAAGGCCTGCAGTAACATGAAATG NGG 10 8 17 8 24 0 100 5 10
    240605172959_r4ug ATACTGAAACAGACTTCCAACAGGAAGGCACG(+CAGTGT)GTACCTGAGAGCAAGTGTTTCTGAATAGAAC NGG 10 8 17 8 24 0 100 5 10
    240605172958_ko3b GTCCAGTTCTATTCAGAAACACTTGCTCTCAG(+CAGTGT)GTACCGTGCCTTCCTGTTGGAAGTCTGTTTC NGG 10 8 17 8 24 0 100 5 10
    240605172956_i3ii TAAATGACAGTGTGTAACTATCTGTAACTGGG(+CAGTGT)GATCCCATGCCCTTTTCTGACCTTCAAGGTC NGG 10 8 17 8 24 0 100 5 10
    240605172954_6svi TTGGTGACCTTGAAGGTCAGAAAAGGGCATGG(+CAGTGT)GATCCCCAGTTACAGATAGTTACACACTGTC NGG 10 8 17 8 24 0 100 5 10
    240605172953_0y73 GCCGTTAGACTGCCTGCCTTTCAGAGGTCTGG(+CAGTGT)GATCCTGGCTCCCCACCCCCAAGTGCGAGAC NGG 10 8 17 8 24 0 100 5 10
    240605172951_whpt AAATGGTCTCGCACTTGGGGGTGGGGAGCCAG(+CAGTGT)GATCCCAGACCTCTGAAAGGCAGGCAGTCTA NGG 10 8 17 8 24 0 100 5 10
    240605172950_6grq TTAGGAACAAGGCAGAGAGCACAGTCGCTCTG(+CAGTGT)GATCCACCCTAAAAAGCCTGCTGACGTGGAG NGG 10 8 17 8 24 0 100 5 10
    240605172948_zim9 TGTCCCTCCACGTCAGCAGGCTTTTTAGGGTG(+CAGTGT)GATCCAGAGCGACTGTGCTCTCTGCCTTGTT NGG 10 8 17 8 24 0 100 5 10
    240605172946_5rkz TCCTCATGTAGCTCACAAGGGCTGGTGTGGAG(+CAGTGT)GATCCGCCTTCTTGCTGGAGAACCCAGCCTC NGG 10 8 17 8 24 0 100 5 10