Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605172931_oabn AAGAAATTAGCTGGTGAATCTTTTCCCTGCTG(+CAGTGT)GATCCTGTGTTCTGGAAGATTAGTCAGGACA NGG 10 8 17 8 24 0 100 5 10
    240605172929_xi6q GGCATGGGTCCGAAGCTCCTTCAATCCTGGTG(+CAGTGT)GATCCGTGGGGAAAGGACGGGGATGGTGAAA NGG 10 8 17 8 24 0 100 5 10
    240605172927_h5r4 CTTGGTTTCACCATCCCCGTCCTTTCCCCACG(+CAGTGT)GATCCACCAGGATTGAAGGAGCTTCGGACCC NGG 10 8 17 8 24 0 100 5 10
    240605172925_2ibs AGGGTCTTATGTAGCCTCACTATGTAGCCAAG(+CAGTGT)GATCCCCTGCCTCCACCTCCCGAGTACAGGG NGG 10 8 17 8 24 0 100 5 10
    240605172923_odmq GTGAGCCCTGTACTCGGGAGGTGGAGGCAGGG(+CAGTGT)GATCCTTGGCTACATAGTGAGGCTACATAAG NGG 10 8 17 8 24 0 100 5 10
    240605172922_ymb9 TACAAGGACACAGATGGTTTGTATTGTCATCG(+CAGTGT)GATCCAGTTGTCATGGACCCATGCCTGGGGA NGG 10 8 17 8 24 0 100 5 10
    240605172921_gfut AGATCTCCCCAGGCATGGGTCCATGACAACTG(+CAGTGT)GATCCGATGACAATACAAACCATCTGTGTCC NGG 10 8 17 8 24 0 100 5 10
    240605172919_cjgc ATCTGATGCCCCTTGCGCTCTCTGAGGGTTGG(+CAGTGT)GTACCATTTTCAATTTCAGAAGAGGACAGAG NGG 10 8 17 8 24 0 100 5 10
    240605172918_guly ATAATCTCTGTCCTCTTCTGAAATTGAAAATG(+CAGTGT)GTACCCAACCCTCAGAGAGCGCAAGGGGCAT NGG 10 8 17 8 24 0 100 5 10
    240605172915_up0h TGTGGACAGTGGGCAGAAGCAGAGGCTCCCTG(+CAGTGT)GATCCTGAACAAGGGCCCCAAAAGCAAGTTC NGG 10 8 17 8 24 0 100 5 10