Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605172828_h4yd CCACCCAAGCACAATCAAGTGTCACAGGACAG(+CAGTGT)GTACCCAACATGCCAACATCCCAGATGGCTC NGG 10 8 17 8 24 0 100 5 10
    240605172827_4yt2 GGAGAGTCACTGGCCTCTCCCGTTGGTTACAG(+CAGTGT)GTACCCTTATACCCTGGATGTTAGCGTTGTA NGG 10 8 17 8 24 0 100 5 10
    240605172825_9o1d ACAACTACAACGCTAACATCCAGGGTATAAGG(+CAGTGT)GTACCTGTAACCAACGGGAGAGGCCAGTGAC NGG 10 8 17 8 24 0 100 5 10
    240605172822_rsd6 CTTGAAAAGCTGGCAGCACTGCAGCCTCCCTG(+CAGTGT)GTACCATTGGTCTGACAGACACAGTTCCAGA NGG 10 8 17 8 24 0 100 5 10
    240605172821_nehs GACTATCTGGAACTGTGTCTGTCAGACCAATG(+CAGTGT)GTACCAGGGAGGCTGCAGTGCTGCCAGCTTT NGG 10 8 17 8 24 0 100 5 10
    240605172818_7m3v CGGAGGATGAAGCCCATCCCTTGCAGATGGGG(+CAGTGT)GTACCCATGCTTTGATGATGTTCACAGAGGG NGG 10 8 17 8 24 0 100 5 10
    240605172816_35wc AGAAGCCCTCTGTGAACATCATCAAAGCATGG(+CAGTGT)GTACCCCCATCTGCAAGGGATGGGCTTCATC NGG 10 8 17 8 24 0 100 5 10
    240605172814_fgof TCGTGTCTATCCAGGCTGGGGGCCAGACTGGG(+CAGTGT)GATCCCTTTCTGGGGTCTGCACTGTGTGTGT NGG 10 8 17 8 24 0 100 5 10
    240605172813_q7p2 CACACACACACACAGTGCAGACCCCAGAAAGG(+CAGTGT)GATCCCCAGTCTGGCCCCCAGCCTGGATAGA NGG 10 8 17 8 24 0 100 5 10
    240605172811_q7rh TGACTTTTCATGGGCGTGCTCTAGGAATCCTG(+CAGTGT)GATCCCACTAAACTTCCATGGCTGGTTGGGC NGG 10 8 17 8 24 0 100 5 10