Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605172718_546c ATCACATGGTGGCTCACAACCATCTGTAATAG(+CAGTGT)GATCCGATGGCCTCTTCTAGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240605172716_y23a CCCTTCCCACTGAGATGCCTGGGCAGCCATGG(+CAGTGT)GTACCCCGAGGCTGATAACACATCAGAGGTG NGG 10 8 17 8 24 0 100 5 10
    240605172714_s023 AATAGCACCTCTGATGTGTTATCAGCCTCGGG(+CAGTGT)GTACCCATGGCTGCCCAGGCATCTCAGTGGG NGG 10 8 17 8 24 0 100 5 10
    240605172712_n2ur GAACCCAGAAGGAGGGTCCAGTGGGCTCTTTG(+CAGTGT)GATCCTTCAGGCTCTTGGAGACGAGAAGCTG NGG 10 8 17 8 24 0 100 5 10
    240605172711_d6sr CTCTCCAGCTTCTCGTCTCCAAGAGCCTGAAG(+CAGTGT)GATCCAAAGAGCCCACTGGACCCTCCTTCTG NGG 10 8 17 8 24 0 100 5 10
    240605172708_kgyp CTACATTCAGGAATATATATACACATTCCTGG(+CAGTGT)GTACCTAGCCACACATGCATACATAAACACA NGG 10 8 17 8 24 0 100 5 10
    240605172707_j6rh GCATGTGTGTTTATGTATGCATGTGTGGCTAG(+CAGTGT)GTACCCAGGAATGTGTATATATATTCCTGAA NGG 10 8 17 8 24 0 100 5 10
    240605172706_szoh CTCCTCCTTTGAGTCATTGCTTCACCCACTAG(+CAGTGT)GATCCTGCCTTACCTTGACACCCAGAAACAA NGG 10 8 17 8 24 0 100 5 10
    240605172704_lvu1 TTGTTTTGTTTCTGGGTGTCAAGGTAAGGCAG(+CAGTGT)GATCCTAGTGGGTGAAGCAATGACTCAAAGG NGG 10 8 17 8 24 0 100 5 10
    240605172703_1o0f AGAAAGGGAGGGATAACCTCTTTCTCAACTAG(+CAGTGT)GTACCAGCTTGAGTCCTTATGTCAAAGGCGA NGG 10 8 17 8 24 0 100 5 10