Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605172629_u1b7 TCCTTCCTTCCTTAAATCACCCCTACTGACAG(+CAGTGT)GATCCTCATGAAACCATTGTCAACTCCGTTC NGG 10 8 17 8 24 0 100 5 10
    240605172628_uvb7 CCCTAGATAGCTATAAAGGAATAGCTAAGGAG(+CAGTGT)GTACCCACATCTCACATGTAGTTCTAGATGA NGG 10 8 17 8 24 0 100 5 10
    240605172626_z0pl ATAATTCATCTAGAACTACATGTGAGATGTGG(+CAGTGT)GTACCTCCTTAGCTATTCCTTTATAGCTATC NGG 10 8 17 8 24 0 100 5 10
    240605172624_tghd GAGCTCAGCACCACTTGCCTGCTAGGGCCTTG(+CAGTGT)GTACCAGTGTGCAGATAGACCTAGCCAAGGC NGG 10 8 17 8 24 0 100 5 10
    240605172623_64bz CCTGTGCCTTGGCTAGGTCTATCTGCACACTG(+CAGTGT)GTACCAAGGCCCTAGCAGGCAAGTGGTGCTG NGG 10 8 17 8 24 0 100 5 10
    240605172622_108p TGTTGAGAGGAAAAATGAAATGGGACAACGTG(+CAGTGT)GTACCAACCAGCTCAGGGCCCACTAAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605172620_0c4i GTGCTCTTCCTTAGTGGGCCCTGAGCTGGTTG(+CAGTGT)GTACCACGTTGTCCCATTTCATTTTTCCTCT NGG 10 8 17 8 24 0 100 5 10
    240605172617_4n17 CACAATCCTGCCCATTCCCCTGTGCCTGGGGG(+CAGTGT)GATCCCCTCCAGAGGTGTTCTCTATCCCGCC NGG 10 8 17 8 24 0 100 5 10
    240605172616_adyb GGGTAGGCGGGATAGAGAACACCTCTGGAGGG(+CAGTGT)GATCCCCCCAGGCACAGGGGAATGGGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240605172614_oes5 TTTGAGCAAGGTTTCCTTCCCAAAGCTTACAG(+CAGTGT)GATCCTGCTTTTGGTATAACTGTAGCTAGCA NGG 10 8 17 8 24 0 100 5 10