Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605172557_vvxv CTCTCTCTAATTCCTGGTTTTGGACTGTGGTG(+CAGTGT)GATCCTGTAGCATAGCCTGGGTAGGGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240605172555_vr4s AGATGTATCCTGAAGCCTTCTTGCCTATCTGG(+CAGTGT)GTACCAGGTGTGACCTCAAGAGATGGCTGCT NGG 10 8 17 8 24 0 100 5 10
    240605172554_c3ct TGTACAGCAGCCATCTCTTGAGGTCACACCTG(+CAGTGT)GTACCCAGATAGGCAAGAAGGCTTCAGGATA NGG 10 8 17 8 24 0 100 5 10
    240605172553_dqrr GGTGACCTCAAAGGTATCCCATGATCAGCATG(+CAGTGT)GTACCAGCAGTAGTGAGTAACCAGTTCTAGT NGG 10 8 17 8 24 0 100 5 10
    240605172551_souv GGGAAACTAGAACTGGTTACTCACTACTGCTG(+CAGTGT)GTACCATGCTGATCATGGGATACCTTTGAGG NGG 10 8 17 8 24 0 100 5 10
    240605172550_f3sq ACGTGATGGTAGGCTTGTGGGGTGACTGCTAG(+CAGTGT)GTACCTCTAGAGGGCAACACTTACACAGGAA NGG 10 8 17 8 24 0 100 5 10
    240605172549_7yux CTGTTTTCCTGTGTAAGTGTTGCCCTCTAGAG(+CAGTGT)GTACCTAGCAGTCACCCCACAAGCCTACCAT NGG 10 8 17 8 24 0 100 5 10
    240605172546_zxmp CAGGTGGTCTGACATCCTCTTGTGCCTCCCTG(+CAGTGT)GTACCTGCAGACACATTCTCTCTCTCTGTCT NGG 10 8 17 8 24 0 100 5 10
    240605172545_6o9n GAGAGAGACAGAGAGAGAGAATGTGTCTGCAG(+CAGTGT)GTACCAGGGAGGCACAAGAGGATGTCAGACC NGG 10 8 17 8 24 0 100 5 10
    240605172544_ij9f GTGTGCACTGCTTGTATGCGTGTGGATGTGTG(+CAGTGT)GATCCCACCTGCCGCCTCCCCATCTGCTCTG NGG 10 8 17 8 24 0 100 5 10