Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605172646_f2st GACAGCCCAGGTCACAGCCAGGGTCAACAGAG(+CAGTGT)GTACCACAGAGCTACCTTTTGGGATGATTGC NGG 10 8 17 8 24 0 100 5 10
    240605172645_ejqj TTCTCTTATCTCTGTTTCAAATCTCCCTGTAG(+CAGTGT)GATCCTGAGGTTACAAACATGTGCCATAGCA NGG 10 8 17 8 24 0 100 5 10
    240605172643_d8r6 CCAGATGCTATGGCACATGTTTGTAACCTCAG(+CAGTGT)GATCCTACAGGGAGATTTGAAACAGAGATAA NGG 10 8 17 8 24 0 100 5 10
    240605172641_p1iz GATCTATCTTGTTATGCAGCCAGAATGGCCTG(+CAGTGT)GATCCCCCTCCTGTGTTTTCAGGTGTTGCTC NGG 10 8 17 8 24 0 100 5 10
    240605172639_lqq9 CAAAAGAGCAACACCTGAAAACACAGGAGGGG(+CAGTGT)GATCCAGGCCATTCTGGCTGCATAACAAGAT NGG 10 8 17 8 24 0 100 5 10
    240605172637_oldk CCCTCCCATAGCAGGCATGCTGACACATCCTG(+CAGTGT)GTACCCTGCCGTACTAACTGCTCTGCTGCCT NGG 10 8 17 8 24 0 100 5 10
    240605172635_tmgw CGTTGAGGCAGCAGAGCAGTTAGTACGGCAGG(+CAGTGT)GTACCAGGATGTGTCAGCATGCCTGCTATGG NGG 10 8 17 8 24 0 100 5 10
    240605172633_8unl CACCTTGCATATGTGAGGCCTTTGCCACCCAG(+CAGTGT)GATCCTCTGACATCTGGGTGATTTCAGATGC NGG 10 8 17 8 24 0 100 5 10
    240605172632_3f0z AAGCTGCATCTGAAATCACCCAGATGTCAGAG(+CAGTGT)GATCCTGGGTGGCAAAGGCCTCACATATGCA NGG 10 8 17 8 24 0 100 5 10
    240605172630_1qa8 GATGAGAACGGAGTTGACAATGGTTTCATGAG(+CAGTGT)GATCCTGTCAGTAGGGGTGATTTAAGGAAGG NGG 10 8 17 8 24 0 100 5 10