Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605171543_dwij AGCAAAGGTTAGGCCTGGAAATGTGAAAACTG(+CAGTGT)GATCCATAACTAAGGATCTAAGAAGTGTAGA NGG 10 8 17 8 24 0 100 5 10
    240605171541_gvwo CTACCCTTGACACAGGAAGTATATCCTAAAGG(+CAGTGT)GATCCGTCGTAGGACACAGCCCTACCCTGGT NGG 10 8 17 8 24 0 100 5 10
    240605171540_3vcl TGTTCACCAGGGTAGGGCTGTGTCCTACGACG(+CAGTGT)GATCCCTTTAGGATATACTTCCTGTGTCAAG NGG 10 8 17 8 24 0 100 5 10
    240605171538_ejb9 CCGGAGGAAACATGAGCGGGATCGTCTGAGCG(+CAGTGT)GATCCCTTTCCCTGGCTGTGTGCGCGTGCGT NGG 10 8 17 8 24 0 100 5 10
    240605171537_fk6m ACTCCACGCACGCGCACACAGCCAGGGAAAGG(+CAGTGT)GATCCGCTCAGACGATCCCGCTCATGTTTCC NGG 10 8 17 8 24 0 100 5 10
    240605171536_v4br AGGAACTCAGGCTGACCTCAGACTCCATGCAG(+CAGTGT)GATCCTCCTGTCAGAGCCATGATGACATAAA NGG 10 8 17 8 24 0 100 5 10
    240605171534_lll1 GTTGCTTTATGTCATCATGGCTCTGACAGGAG(+CAGTGT)GATCCTGCATGGAGTCTGAGGTCAGCCTGAG NGG 10 8 17 8 24 0 100 5 10
    240605171532_0dz2 CTGAGAACTTGAGACAAGGCTTACCACAGGAG(+CAGTGT)GTACCCACTCCCCCATACCTCCTGGACAAGC NGG 10 8 17 8 24 0 100 5 10
    240605171530_i5d4 TGTCTGCTTGTCCAGGAGGTATGGGGGAGTGG(+CAGTGT)GTACCTCCTGTGGTAAGCCTTGTCTCAAGTT NGG 10 8 17 8 24 0 100 5 10
    240605171529_607d GAATCAGATAACAGAATCAGTGTTTCAGTCAG(+CAGTGT)GATCCACACTGCCTGCCAGGCTGTGCTGTGG NGG 10 8 17 8 24 0 100 5 10