Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605171557_rzmc TAAAACTCAAGTATGGAATCCTCCCCCATAGG(+CAGTGT)GATCCTGCCCAGTGGGTCCATGATTTAGGAA NGG 10 8 17 8 24 0 100 5 10
    240605171556_z37z GATAAAAACTAAGAACAGAAACCCCACGAAAG(+CAGTGT)GATCCTACTTCTCGTCTCTCATTGCAGGATA NGG 10 8 17 8 24 0 100 5 10
    240605171554_one5 AGATATATCCTGCAATGAGAGACGAGAAGTAG(+CAGTGT)GATCCTTTCGTGGGGTTTCTGTTCTTAGTTT NGG 10 8 17 8 24 0 100 5 10
    240605171552_ird6 TATTGAATGCATTGTATTAAAGAAGCACCATG(+CAGTGT)GTACCTCCTGTTTGAAGTTAATTGTTCATGC NGG 10 8 17 8 24 0 100 5 10
    240605171551_jxxy AATGTGCATGAACAATTAACTTCAAACAGGAG(+CAGTGT)GTACCATGGTGCTTCTTTAATACAATGCATT NGG 10 8 17 8 24 0 100 5 10
    240605171549_v7bb TATTGAATGCATTGTATTAAAGAAGCACCATG(+CAGTGT)GTACCTCCTGTTTGAAGTTAATTGTTCATGC NGG 10 8 17 8 24 0 100 5 10
    240605171547_fk0i AATGTGCATGAACAATTAACTTCAAACAGGAG(+CAGTGT)GTACCATGGTGCTTCTTTAATACAATGCATT NGG 10 8 17 8 24 0 100 5 10
    240605171546_p298 CTTAATGCCCAGTGTGTTTGGCAATATTTCAG(+CAGTGT)GATCCGAAAATTATTCAAATTTTGTTTGTTA NGG 10 8 17 8 24 0 100 5 10
    240605171545_0ph9 TTTAATAACAAACAAAATTTGAATAATTTTCG(+CAGTGT)GATCCTGAAATATTGCCAAACACACTGGGCA NGG 10 8 17 8 24 0 100 5 10
    240605171544_3tmj ACATGTCTACACTTCTTAGATCCTTAGTTATG(+CAGTGT)GATCCAGTTTTCACATTTCCAGGCCTAACCT NGG 10 8 17 8 24 0 100 5 10