Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605171655_ox11 ATCAACACACCATGTATGGATTCTGGAGAGTG(+CAGTGT)GTACCAGCAGGGAACCCACCGGATGAAGGAA NGG 10 8 17 8 24 0 100 5 10
    240605171654_kcsa GTTTCTAGATGAAAGGGGATTTCTATTCAGAG(+CAGTGT)GTACCTGTGTGTATCTGTTAGTAACCCCAGG NGG 10 8 17 8 24 0 100 5 10
    240605171653_k0g6 TAGTCCCTGGGGTTACTAACAGATACACACAG(+CAGTGT)GTACCTCTGAATAGAAATCCCCTTTCATCTA NGG 10 8 17 8 24 0 100 5 10
    240605171651_662o GGGAAGTCATGGCATGTTACTGCAAAGGTCTG(+CAGTGT)GTACCCTCAACTCATAGAGTTGACAGGTTAG NGG 10 8 17 8 24 0 100 5 10
    240605171649_k8vj TGTCCCTAACCTGTCAACTCTATGAGTTGAGG(+CAGTGT)GTACCAGACCTTTGCAGTAACATGCCATGAC NGG 10 8 17 8 24 0 100 5 10
    240605171648_0zbq GGTCAGGTTGTCACAGGAATACCCCATTCCTG(+CAGTGT)GTACCAATCTGTCTTAGTCAGGGTTTCTATT NGG 10 8 17 8 24 0 100 5 10
    240605171646_9cea GCAGGAATAGAAACCCTGACTAAGACAGATTG(+CAGTGT)GTACCAGGAATGGGGTATTCCTGTGACAACC NGG 10 8 17 8 24 0 100 5 10
    240605171645_aacf ATGGAACCAGCGTCTGTGAAAAGTCAGGATTG(+CAGTGT)GTACCAACACGTGTGAGTGAAAACTCTGGGA NGG 10 8 17 8 24 0 100 5 10
    240605171644_mj8g AGAAATCCCAGAGTTTTCACTCACACGTGTTG(+CAGTGT)GTACCAATCCTGACTTTTCACAGACGCTGGT NGG 10 8 17 8 24 0 100 5 10
    240605171643_2sog CAGCCTGGGAAGGTGGGAGGCAGAGAACACAG(+CAGTGT)GTACCTCCCTGGAAGTACATGAGCTATCTGG NGG 10 8 17 8 24 0 100 5 10