Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605171710_vja4 AAAAAAAAGAAGGACAAAGCAATGGGGATTTG(+CAGTGT)GTACCCTTTTACTTAAGTGGACTGTGTTATA NGG 10 8 17 8 24 0 100 5 10
    240605171709_2rkq ACTACATCCATGTTAAAATCCGTGTAGTCAGG(+CAGTGT)GATCCTGTAAGTCACTCCCTATGAAGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240605171708_b7yn CCCAATCCTGCTTCATAGGGAGTGACTTACAG(+CAGTGT)GATCCCTGACTACACGGATTTTAACATGGAT NGG 10 8 17 8 24 0 100 5 10
    240605171706_lgax CTTGCAGGTGCCCTTGGAGGCCAGGAACGTTG(+CAGTGT)GATCCCCTGGGACCTGATATTACAGGTGGCT NGG 10 8 17 8 24 0 100 5 10
    240605171704_q7f6 CTGACAGCCACCTGTAATATCAGGTCCCAGGG(+CAGTGT)GATCCAACGTTCCTGGCCTCCAAGGGCACCT NGG 10 8 17 8 24 0 100 5 10
    240605171702_hkjv CCCCAGGTGCCTAAATTCCAGGAGAATCACAG(+CAGTGT)GTACCAGTCTCAATTCACCACTTAACTTTAG NGG 10 8 17 8 24 0 100 5 10
    240605171701_bo6m ACTTCCTAAAGTTAAGTGGTGAATTGAGACTG(+CAGTGT)GTACCTGTGATTCTCCTGGAATTTAGGCACC NGG 10 8 17 8 24 0 100 5 10
    240605171659_4x31 CCATCCTGGGAAAGTGACTGGGGCCCACGTGG(+CAGTGT)GATCCTAGAGCCTAGCACAGTGATATGTCCA NGG 10 8 17 8 24 0 100 5 10
    240605171658_pvj9 TGCTTTGGACATATCACTGTGCTAGGCTCTAG(+CAGTGT)GATCCCACGTGGGCCCCAGTCACTTTCCCAG NGG 10 8 17 8 24 0 100 5 10
    240605171657_hk3x TGTACTTCCTTCATCCGGTGGGTTCCCTGCTG(+CAGTGT)GTACCACTCTCCAGAATCCATACATGGTGTG NGG 10 8 17 8 24 0 100 5 10