Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605171758_j6rn TGTTTTCCATGAAAGCCCGAGGACCTCTCTTG(+CAGTGT)GATCCCAGCATCCACAGAAGACGCCTAGTGA NGG 10 8 17 8 24 0 100 5 10
    240605171757_1qsw ATCAGCATTGGGGTACTGACAGAAACCATAAG(+CAGTGT)GATCCTGCCCTCCACTGTTCCTTGGTTCCTA NGG 10 8 17 8 24 0 100 5 10
    240605171755_e0en GGACATAGGAACCAAGGAACAGTGGAGGGCAG(+CAGTGT)GATCCTTATGGTTTCTGTCAGTACCCCAATG NGG 10 8 17 8 24 0 100 5 10
    240605171754_7wp2 TGAGATTAATCTTTTAAAGTAGATTAACAATG(+CAGTGT)GATCCTTTGGAGCTGGAGTTACAGATGGTTG NGG 10 8 17 8 24 0 100 5 10
    240605171752_um3m GCTCACAACCATCTGTAACTCCAGCTCCAAAG(+CAGTGT)GATCCATTGTTAATCTACTTTAAAAGATTAA NGG 10 8 17 8 24 0 100 5 10
    240605171751_87ix GGTGTCAGCATTTGGAAGCTGATTATGGGATG(+CAGTGT)GATCCCTGCATATGGCAATCACTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605171749_mmd2 GGATGGACCATCTAGTGATTGCCATATGCAGG(+CAGTGT)GATCCATCCCATAATCAGCTTCCAAATGCTG NGG 10 8 17 8 24 0 100 5 10
    240605171747_csu9 TTCTGGATATATGCCCAGGAGAGGTATTGCGG(+CAGTGT)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605171745_t5h0 TCCTCAGAAAATTGGACATAGTACTACCGGAG(+CAGTGT)GATCCCGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240605171744_0cwu GGGACACAGAAACAGGGGAGCAGATGGGACAG(+CAGTGT)GATCCTTTCAGCACCACCTATACCTGGAGGT NGG 10 8 17 8 24 0 100 5 10