Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605171842_njt6 TCATGCTCAAAGAGGAGAGCACAGGACACTCG(+CAGTGT)GTACCCCATAAGCTATGCCAAGCTCCCAGCT NGG 10 8 17 8 24 0 100 5 10
    240605171840_4ivf TGTCACCAGCTGCCCTTCTCACCAGGAAACAG(+CAGTGT)GTACCTTTCCCTCTGCCTAGCGCCAACCCCA NGG 10 8 17 8 24 0 100 5 10
    240605171839_zphc CATTGTGGGGTTGGCGCTAGGCAGAGGGAAAG(+CAGTGT)GTACCTGTTTCCTGGTGAGAAGGGCAGCTGG NGG 10 8 17 8 24 0 100 5 10
    240605171837_qkud CAACCGGCCGGAGCAGCTGGGGCAGAGGATGG(+CAGTGT)GTACCACAGGTTGAGGGGATGAAGGTCTGAT NGG 10 8 17 8 24 0 100 5 10
    240605171835_s068 CCCTCATCAGACCTTCATCCCCTCAACCTGTG(+CAGTGT)GTACCCATCCTCTGCCCCAGCTGCTCCGGCC NGG 10 8 17 8 24 0 100 5 10
    240605171834_93hr AACCAGGCAGCGGCTCTCTCGATGACATTCAG(+CAGTGT)GATCCCTAAAGTCATATGCTTCCCACTATAG NGG 10 8 17 8 24 0 100 5 10
    240605171832_o5rx GGAAGCTATAGTGGGAAGCATATGACTTTAGG(+CAGTGT)GATCCTGAATGTCATCGAGAGAGCCGCTGCC NGG 10 8 17 8 24 0 100 5 10
    240605171831_670e GGTAGATCTGGGAAAAGGTAGGGGAGGAATAG(+CAGTGT)GATCCAGATCAAAATACACTGTGAGTATGAA NGG 10 8 17 8 24 0 100 5 10
    240605171830_owh9 TATCATTCATACTCACAGTGTATTTTGATCTG(+CAGTGT)GATCCTATTCCTCCCCTACCTTTTCCCAGAT NGG 10 8 17 8 24 0 100 5 10
    240605171829_llfl TTACATAAAAGGCTGACAAGGCCTTGGCTCTG(+CAGTGT)GTACCAAATGGTCCTCTGCCTGGAATGCCTT NGG 10 8 17 8 24 0 100 5 10