Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605171946_2a3c GTTGTTCTCAGAGAAATATCATTGAGGTGGGG(+CAGTGT)GTACCTCGTCCCAGGGTGCTCAAAGTTCTCA NGG 10 8 17 8 24 0 100 5 10
    240605171945_n8h1 TTCTAATTCCTGGAAATTGTGTGGGTGGAGTG(+CAGTGT)GATCCCTGCACTGGGGGGAATAACATGGACC NGG 10 8 17 8 24 0 100 5 10
    240605171943_w2q7 TCAGTGGTCCATGTTATTCCCCCCAGTGCAGG(+CAGTGT)GATCCACTCCACCCACACAATTTCCAGGAAT NGG 10 8 17 8 24 0 100 5 10
    240605171942_d7el GAAAACCACTAAGAATCTTCCAGCACTGTATG(+CAGTGT)GATCCTCAATGTTTCTATCTTATTGTAAAGG NGG 10 8 17 8 24 0 100 5 10
    240605171941_fhvw CATTTCCTTTACAATAAGATAGAAACATTGAG(+CAGTGT)GATCCATACAGTGCTGGAAGATTCTTAGTGG NGG 10 8 17 8 24 0 100 5 10
    240605171939_dqb5 GAAAACCACTAAGAATCTTCCAGCACTGTATG(+CAGTGT)GATCCTCAATGTTTCTATCTTATTGTAAAGG NGG 10 8 17 8 24 0 100 5 10
    240605171938_li7n CATTTCCTTTACAATAAGATAGAAACATTGAG(+CAGTGT)GATCCATACAGTGCTGGAAGATTCTTAGTGG NGG 10 8 17 8 24 0 100 5 10
    240605171937_1n7b GAAAACCACTAAGAATCTTCCAGCACTGTATG(+CAGTGT)GATCCTCAATGTTTCTATCTTATTGTAAAGG NGG 10 8 17 8 24 0 100 5 10
    240605171935_0fn6 CATTTCCTTTACAATAAGATAGAAACATTGAG(+CAGTGT)GATCCATACAGTGCTGGAAGATTCTTAGTGG NGG 10 8 17 8 24 0 100 5 10
    240605171934_4uj7 GAAAACCACTAAGAATCTTCCAGCACTGTATG(+CAGTGT)GATCCTCAATGTTTCTATCTTATTGTAAAGG NGG 10 8 17 8 24 0 100 5 10