Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605172032_iced CTGTGGAATGAACCACCACAGAAAGATCCACG(+CAGTGT)GTACCATACACATTCTTTTAAGTTTTCTTGT NGG 10 8 17 8 24 0 100 5 10
    240605172031_0vbo ACTGAAAAGAAAAGGAATGAAGAAGACTTCTG(+CAGTGT)GATCCAAAGCCTTAAGAGAGTCTACGTGTGC NGG 10 8 17 8 24 0 100 5 10
    240605172030_9xa6 TGTTAGCACACGTAGACTCTCTTAAGGCTTTG(+CAGTGT)GATCCAGAAGTCTTCTTCATTCCTTTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240605172028_zp2a TGCGTTCCTGCAGTGTCCTCCTCGAGGAGCAG(+CAGTGT)GATCCACATTTTCAAGAATGTCCTGACTCCT NGG 10 8 17 8 24 0 100 5 10
    240605172027_dmi7 TCACGAGGAGTCAGGACATTCTTGAAAATGTG(+CAGTGT)GATCCTGCTCCTCGAGGAGGACACTGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240605172026_dz4v AGCACATGTCAAGAAGGACTCAGGAAAGAAAG(+CAGTGT)GTACCTGTGCCTTGCCCCAGTGTCTGTCTGA NGG 10 8 17 8 24 0 100 5 10
    240605172024_q44u TGAACTCAGACAGACACTGGGGCAAGGCACAG(+CAGTGT)GTACCTTTCTTTCCTGAGTCCTTCTTGACAT NGG 10 8 17 8 24 0 100 5 10
    240605172023_bxxv ATACCTTCCAAGGACCAGTAGACTTGAATACG(+CAGTGT)GATCCAATGGGAGTTGGTTGTCTGGTGTCAA NGG 10 8 17 8 24 0 100 5 10
    240605172021_faki TCATCTTGACACCAGACAACCAACTCCCATTG(+CAGTGT)GATCCGTATTCAAGTCTACTGGTCCTTGGAA NGG 10 8 17 8 24 0 100 5 10
    240605172019_v5ua GACTCCCTCCAGGGGTTCCCAGCTCCCACAGG(+CAGTGT)GTACCTGCCATCTCCATCCCTGGACTGCAGA NGG 10 8 17 8 24 0 100 5 10