Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605172109_j6z8 GGCATTTAAATCCAAGCCACACAAGATCCTGG(+CAGTGT)GTACCTTTTAAACTAGGGAGAGCACTGCCCG NGG 10 8 17 8 24 0 100 5 10
    240605172108_4bm2 GAGTGAAAACAAGCTTTTCTTCAAATCTTCAG(+CAGTGT)GTACCTACCTCAGAATAGGTGTCATTAACGT NGG 10 8 17 8 24 0 100 5 10
    240605172107_em8g TGAAAACGTTAATGACACCTATTCTGAGGTAG(+CAGTGT)GTACCTGAAGATTTGAAGAAAAGCTTGTTTT NGG 10 8 17 8 24 0 100 5 10
    240605172105_v0jk CTCGAGGAATAGAAACACTGGATACTTAATGG(+CAGTGT)GTACCAGAAGAGTTTAGGAAACATGGAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605172104_csar AGCCACTCCTCCATGTTTCCTAAACTCTTCTG(+CAGTGT)GTACCCATTAAGTATCCAGTGTTTCTATTCC NGG 10 8 17 8 24 0 100 5 10
    240605172102_xnr1 TGGCAGTTTGATGGCAGTACTCTGTGTAGCAG(+CAGTGT)GATCCTGGATGAGACGAGGAGGAATCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240605172100_giyg CATGGCTGCAGATTCCTCCTCGTCTCATCCAG(+CAGTGT)GATCCTGCTACACAGAGTACTGCCATCAAAC NGG 10 8 17 8 24 0 100 5 10
    240605172058_67qt GAGCTTAAAAGACACTCGTTAGAGTGCACCTG(+CAGTGT)GATCCTGCCATGACATCACAGGCGAAGCATC NGG 10 8 17 8 24 0 100 5 10
    240605172057_o54t CGGCTGATGCTTCGCCTGTGATGTCATGGCAG(+CAGTGT)GATCCAGGTGCACTCTAACGAGTGTCTTTTA NGG 10 8 17 8 24 0 100 5 10
    240605172056_cgvh AAATGACCAGGCTCCTGGTAAATGGAAATCAG(+CAGTGT)GATCCAAGCAACTTTGAATTCTGGGAAATGA NGG 10 8 17 8 24 0 100 5 10