Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605172125_yha2 ACAGCATCAAGATAATAGTTGTCTTGGAACTG(+CAGTGT)GATCCAATTTTTTAGTTTCTGTCCTCTCATA NGG 10 8 17 8 24 0 100 5 10
    240605172123_aybw CCCTATGTCTCCACTCGCAATCATATACTTAG(+CAGTGT)GTACCTTTGACCCCATGGAAAAAATATCTAA NGG 10 8 17 8 24 0 100 5 10
    240605172122_0cby GAATGTTAGATATTTTTTCCATGGGGTCAAAG(+CAGTGT)GTACCTAAGTATATGATTGCGAGTGGAGACA NGG 10 8 17 8 24 0 100 5 10
    240605172120_xi11 CTGTGTATCTTTAAATACATCTGGATTGTGGG(+CAGTGT)GTACCTATGCGCAGTCTAGTTCAAAAATAAA NGG 10 8 17 8 24 0 100 5 10
    240605172119_z6ce ATTTATTTATTTTTGAACTAGACTGCGCATAG(+CAGTGT)GTACCCCACAATCCAGATGTATTTAAAGATA NGG 10 8 17 8 24 0 100 5 10
    240605172118_el7i ACACTGTGCCCTAGTTCTGTGAGAATGAGCAG(+CAGTGT)GTACCTCTCTTCCCAGAGGCCATCACCTAGA NGG 10 8 17 8 24 0 100 5 10
    240605172116_ebl7 TCCCATCTAGGTGATGGCCTCTGGGAAGAGAG(+CAGTGT)GTACCTGCTCATTCTCACAGAACTAGGGCAC NGG 10 8 17 8 24 0 100 5 10
    240605172114_xwhm GGATTTCAAAGTGATGTCCGATGTAGCGCCAG(+CAGTGT)GTACCTTTGAATATCAATCAGAACCATTTAG NGG 10 8 17 8 24 0 100 5 10
    240605172113_s0xo CATATCTAAATGGTTCTGATTGATATTCAAAG(+CAGTGT)GTACCTGGCGCTACATCGGACATCACTTTGA NGG 10 8 17 8 24 0 100 5 10
    240605172112_8vaa TGTTTCGGGCAGTGCTCTCCCTAGTTTAAAAG(+CAGTGT)GTACCCAGGATCTTGTGTGGCTTGGATTTAA NGG 10 8 17 8 24 0 100 5 10