Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605172018_mc6o GTGGCTCTGCAGTCCAGGGATGGAGATGGCAG(+CAGTGT)GTACCCTGTGGGAGCTGGGAACCCCTGGAGG NGG 10 8 17 8 24 0 100 5 10
    240605172016_pwkt ATTTTGGAAGAACACAGAAAGTTTTCAGACAG(+CAGTGT)GATCCATTTCCCCAGTACTCAACCAAAATTG NGG 10 8 17 8 24 0 100 5 10
    240605172015_4dzx CTTTCCAATTTTGGTTGAGTACTGGGGAAATG(+CAGTGT)GATCCTGTCTGAAAACTTTCTGTGTTCTTCC NGG 10 8 17 8 24 0 100 5 10
    240605172012_pex0 CCAAGAGGATGGGATTCATATTTAGTCCCTAG(+CAGTGT)GTACCGTTAAGAAGTGGCACACTATTGTAAG NGG 10 8 17 8 24 0 100 5 10
    240605172011_11n4 ATATACTTACAATAGTGTGCCACTTCTTAACG(+CAGTGT)GTACCTAGGGACTAAATATGAATCCCATCCT NGG 10 8 17 8 24 0 100 5 10
    240605172009_964i TCATGCAGTGTCACCCGTGTCACGCAGCGCGG(+CAGTGT)GTACCCTGAGAGGACTCTGAATACCCTTCCC NGG 10 8 17 8 24 0 100 5 10
    240605172008_d2ty AGTTGGGGAAGGGTATTCAGAGTCCTCTCAGG(+CAGTGT)GTACCCGCGCTGCGTGACACGGGTGACACTG NGG 10 8 17 8 24 0 100 5 10
    240605172006_fb4m TCATTTTGTTTCCTTTTAAGAAATTGCCACAG(+CAGTGT)GTACCCCTACGTTTAGTAACTACCATGCTGA NGG 10 8 17 8 24 0 100 5 10
    240605172004_opcy ACTTATCAGCATGGTAGTTACTAAACGTAGGG(+CAGTGT)GTACCTGTGGCAATTTCTTAAAAGGAAACAA NGG 10 8 17 8 24 0 100 5 10
    240605172003_ohlg TATATTTCCCTTTCCCATTCAATCAATAGGTG(+CAGTGT)GATCCAAGCTTGTTAGCTACTCAGGACTTGC NGG 10 8 17 8 24 0 100 5 10