Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605171933_usav CATTTCCTTTACAATAAGATAGAAACATTGAG(+CAGTGT)GATCCATACAGTGCTGGAAGATTCTTAGTGG NGG 10 8 17 8 24 0 100 5 10
    240605171931_otd4 AAAAACCACTAAGAATCTTCCAGCACTGTATG(+CAGTGT)GATCCTTAATGTCTCTATCTTATTGTAAAGG NGG 10 8 17 8 24 0 100 5 10
    240605171930_16sg CATTTCCTTTACAATAAGATAGAGACATTAAG(+CAGTGT)GATCCATACAGTGCTGGAAGATTCTTAGTGG NGG 10 8 17 8 24 0 100 5 10
    240605171928_wnrr CCAGGGTGACAACTTCATCTCCCATCCATGTG(+CAGTGT)GTACCAGTCTTACCATTCTCTTCATAAGCTC NGG 10 8 17 8 24 0 100 5 10
    240605171926_ywzz ATCCAGAGCTTATGAAGAGAATGGTAAGACTG(+CAGTGT)GTACCACATGGATGGGAGATGAAGTTGTCAC NGG 10 8 17 8 24 0 100 5 10
    240605171925_ksqj TTACATTAACAAGGCAAAATAAAGCCACAATG(+CAGTGT)GTACCATTAGGAAATTCCATCAAAACAACAG NGG 10 8 17 8 24 0 100 5 10
    240605171924_qcct AAAGTCTGTTGTTTTGATGGAATTTCCTAATG(+CAGTGT)GTACCATTGTGGCTTTATTTTGCCTTGTTAA NGG 10 8 17 8 24 0 100 5 10
    240605171922_d5ae TGGTGTGATAGTTTCTTAGGAGCAAACAACAG(+CAGTGT)GATCCTTTCTCCTAAACACAGGTTTAATTTT NGG 10 8 17 8 24 0 100 5 10
    240605171921_zxsh TGTTGAAAATTAAACCTGTGTTTAGGAGAAAG(+CAGTGT)GATCCTGTTGTTTGCTCCTAAGAAACTATCA NGG 10 8 17 8 24 0 100 5 10
    240605171919_qv5b GCATGGGATGTCCTATTTTAGGAGGTTTCCAG(+CAGTGT)GATCCTAGCCCACTAAATTAACATCATGCCA NGG 10 8 17 8 24 0 100 5 10