Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605171827_pos0 GAGAGAAGGCATTCCAGGCAGAGGACCATTTG(+CAGTGT)GTACCAGAGCCAAGGCCTTGTCAGCCTTTTA NGG 10 8 17 8 24 0 100 5 10
    240605171826_l0mr GGCTCTATCTACTGAAATGAAACACACTGATG(+CAGTGT)GTACCTGGATTTCCACACCTAGTTACTTATC NGG 10 8 17 8 24 0 100 5 10
    240605171824_bv83 TTATCGATAAGTAACTAGGTGTGGAAATCCAG(+CAGTGT)GTACCATCAGTGTGTTTCATTTCAGTAGATA NGG 10 8 17 8 24 0 100 5 10
    240605171823_64gu AATCAAATCAGCCCTAAACTGTCTCTTTCTTG(+CAGTGT)GATCCTGAGCATAGATTTACCATCTAGAACC NGG 10 8 17 8 24 0 100 5 10
    240605171822_1yh0 ATCTGGGTTCTAGATGGTAAATCTATGCTCAG(+CAGTGT)GATCCAAGAAAGAGACAGTTTAGGGCTGATT NGG 10 8 17 8 24 0 100 5 10
    240605171821_tez2 TGTGGAGATTTCTCCACTAGAAAACTGTAAAG(+CAGTGT)GTACCCACATTTCCTCCTGAGACTATCACCT NGG 10 8 17 8 24 0 100 5 10
    240605171819_zxj5 GAGACAGGTGATAGTCTCAGGAGGAAATGTGG(+CAGTGT)GTACCTTTACAGTTTTCTAGTGGAGAAATCT NGG 10 8 17 8 24 0 100 5 10
    240605171818_js74 TGCCTCAGTCCTTCACTGCAACTCCCCAGTAG(+CAGTGT)GATCCCCACAGTTGTTTTTGTGCCTCCCTCG NGG 10 8 17 8 24 0 100 5 10
    240605171816_2272 AAACACGAGGGAGGCACAAAAACAACTGTGGG(+CAGTGT)GATCCTACTGGGGAGTTGCAGTGAAGGACTG NGG 10 8 17 8 24 0 100 5 10
    240605171814_qmob AACTAAGAAACTTGCACTTACATGTCGAATGG(+CAGTGT)GTACCATCTAATAGCTAACACCCTGTAGCAG NGG 10 8 17 8 24 0 100 5 10