Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605171812_3qg3 TTGGCCTGCTACAGGGTGTTAGCTATTAGATG(+CAGTGT)GTACCCATTCGACATGTAAGTGCAAGTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605171811_jf5z GACAAGCAAAGGAGCTGGGGAGTGGAGTGTAG(+CAGTGT)GTACCATCACCTGAAAGCCACAGTCCTGACA NGG 10 8 17 8 24 0 100 5 10
    240605171810_faym TTGTCTGTCAGGACTGTGGCTTTCAGGTGATG(+CAGTGT)GTACCTACACTCCACTCCCCAGCTCCTTTGC NGG 10 8 17 8 24 0 100 5 10
    240605171808_z1mr TGTTCACAGCAGTAAAACCCTGACTGAGACAG(+CAGTGT)GTACCATCTAATAGCTAACACAATGTCACAG NGG 10 8 17 8 24 0 100 5 10
    240605171807_wwy4 CTTGCCTGTGACATTGTGTTAGCTATTAGATG(+CAGTGT)GTACCTGTCTCAGTCAGGGTTTTACTGCTGT NGG 10 8 17 8 24 0 100 5 10
    240605171806_1hr1 GGGACACAGAAACAGGGGAGCAGATGGGACAG(+CAGTGT)GATCCTTTCAGTCACCATCTACACCTGGAGG NGG 10 8 17 8 24 0 100 5 10
    240605171804_d402 CACAACCTCCAGGTGTAGATGGTGACTGAAAG(+CAGTGT)GATCCTGTCCCATCTGCTCCCCTGTTTCTGT NGG 10 8 17 8 24 0 100 5 10
    240605171803_puet GTGCTCTCAGTCAACCACTGGACTAAGCACAG(+CAGTGT)GATCCCCAGTGGAGGAGTTAGAGAAAGGCCT NGG 10 8 17 8 24 0 100 5 10
    240605171801_xf52 CCTTCAGGCCTTTCTCTAACTCCTCCACTGGG(+CAGTGT)GATCCTGTGCTTAGTCCAGTGGTTGACTGAG NGG 10 8 17 8 24 0 100 5 10
    240605171800_4coi TTGCATCACTAGGCGTCTTCTGTGGATGCTGG(+CAGTGT)GATCCAAGAGAGGTCCTCGGGCTTTCATGGA NGG 10 8 17 8 24 0 100 5 10