Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605171641_6ycn CTTTGCCAGATAGCTCATGTACTTCCAGGGAG(+CAGTGT)GTACCTGTGTTCTCTGCCTCCCACCTTCCCA NGG 10 8 17 8 24 0 100 5 10
    240605171640_p0jn ATGATGGTGCTTGGTGAGTCGTGGTTCTAAAG(+CAGTGT)GTACCAAACGGGGGAGCCAAAGTTCTCCAAC NGG 10 8 17 8 24 0 100 5 10
    240605171638_2b7l CAGCAGTTGGAGAACTTTGGCTCCCCCGTTTG(+CAGTGT)GTACCTTTAGAACCACGACTCACCAAGCACC NGG 10 8 17 8 24 0 100 5 10
    240605171637_7k89 AATAACTTTAATTTACATACTCACTCACACAG(+CAGTGT)GTACCAGTGCTTTGAAAATAGATTGTTCAGT NGG 10 8 17 8 24 0 100 5 10
    240605171636_1xe8 TTAGGACTGAACAATCTATTTTCAAAGCACTG(+CAGTGT)GTACCTGTGTGAGTGAGTATGTAAATTAAAG NGG 10 8 17 8 24 0 100 5 10
    240605171635_cjht TTGTGTTTAAACCCTTGGCTTCAAGGAGTCAG(+CAGTGT)GATCCCCCTATCTTTCATCAAGTTTCAGGAA NGG 10 8 17 8 24 0 100 5 10
    240605171632_inu3 AGTGTTTCCTGAAACTTGATGAAAGATAGGGG(+CAGTGT)GATCCTGACTCCTTGAAGCCAAGGGTTTAAA NGG 10 8 17 8 24 0 100 5 10
    240605171631_ik82 TCTCGGATACAATCGATGCGTCTGACCTTTGG(+CAGTGT)GATCCTCACCATATGGGCAAACAATGGGCTT NGG 10 8 17 8 24 0 100 5 10
    240605171629_4m8p CTGCAAAGCCCATTGTTTGCCCATATGGTGAG(+CAGTGT)GATCCCAAAGGTCAGACGCATCGATTGTATC NGG 10 8 17 8 24 0 100 5 10
    240605171628_a838 AATAATGTTAACATTATTATATAAACAACTTG(+CAGTGT)GATCCCAAATTCTTGGAAGTTCATGTGTAAA NGG 10 8 17 8 24 0 100 5 10