Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605171422_lt1s CTGTGGGGACCACAACTTGCTTGCTAAGCCTG(+CAGTGT)GTACCCATACCTCATAGCTCCCATTGAAAGT NGG 10 8 17 8 24 0 100 5 10
    240605171420_86ar TTGTTCAGGGACTACTCAAGAAAATATCTTTG(+CAGTGT)GATCCTTAGTTGGCCTTCAAGTCTATACATA NGG 10 8 17 8 24 0 100 5 10
    240605171419_5su7 TCTTATATGTATAGACTTGAAGGCCAACTAAG(+CAGTGT)GATCCAAAGATATTTTCTTGAGTAGTCCCTG NGG 10 8 17 8 24 0 100 5 10
    240605171416_hpdg ACACAGTTTGAAGCACTATGTTTCTTCCCCTG(+CAGTGT)GTACCCTCTCTGTTCACAACTGCAGAAGCCT NGG 10 8 17 8 24 0 100 5 10
    240605171414_bu23 GAGAGAGGCTTCTGCAGTTGTGAACAGAGAGG(+CAGTGT)GTACCAGGGGAAGAAACATAGTGCTTCAAAC NGG 10 8 17 8 24 0 100 5 10
    240605171412_pdp6 GTCATTGATGGGACAGGACAGGGAGATGGGAG(+CAGTGT)GATCCCCCAAACACTAGGAAGCAAGCAGCAG NGG 10 8 17 8 24 0 100 5 10
    240605171410_mlum TGTTTCTGCTGCTTGCTTCCTAGTGTTTGGGG(+CAGTGT)GATCCTCCCATCTCCCTGTCCTGTCCCATCA NGG 10 8 17 8 24 0 100 5 10
    240605171408_yw7r TCATCTGGTTGAAAACAGAAATCCAGAACCTG(+CAGTGT)GATCCCTAGGTCTTAGGGCTGACCCACACCA NGG 10 8 17 8 24 0 100 5 10
    240605171406_fewh GAACATGGTGTGGGTCAGCCCTAAGACCTAGG(+CAGTGT)GATCCAGGTTCTGGATTTCTGTTTTCAACCA NGG 10 8 17 8 24 0 100 5 10
    240605171405_was2 CTAGACTGCAGAGCTTCCAATAAAGCCGCTTG(+CAGTGT)GTACCGTGGATCTGTCTGTCAGCCTCTGTTC NGG 10 8 17 8 24 0 100 5 10