Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605171333_lb88 GCTTCAAAAATAAGGTAGAGAATGATTTGAGG(+CAGTGT)GTACCTGGTAACATCTGTCCTGCACACACAG NGG 10 8 17 8 24 0 100 5 10
    240605171331_m657 AGCACCTTCCTGGAGCCCCGTGCCCTTGCTGG(+CAGTGT)GATCCTGAGACAACTGTCAGCTGAGCCCTGT NGG 10 8 17 8 24 0 100 5 10
    240605171330_jww1 TTCCCACAGGGCTCAGCTGACAGTTGTCTCAG(+CAGTGT)GATCCCAGCAAGGGCACGGGGCTCCAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605171327_davs ACAGACCAGGCAGGGCTGGCTGGAGTTGAGGG(+CAGTGT)GATCCCGGTCCAAGAGAGAGAGAGCGCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605171325_tuqk CAGCGCCCAGCGCTCTCTCTCTCTTGGACCGG(+CAGTGT)GATCCCCTCAACTCCAGCCAGCCCTGCCTGG NGG 10 8 17 8 24 0 100 5 10
    240605171323_7ksu CGAGAGGGCGTCAAAGAGGAAGCCTGGGCTGG(+CAGTGT)GATCCTTAGGCTCAGCTGTCTGTCTAATCCC NGG 10 8 17 8 24 0 100 5 10
    240605171321_b8yg GCTCTGGGATTAGACAGACAGCTGAGCCTAAG(+CAGTGT)GATCCCAGCCCAGGCTTCCTCTTTGACGCCC NGG 10 8 17 8 24 0 100 5 10
    240605171320_ieqt GCTGTCTTTAGACACACCAGAAGAGGACATTG(+CAGTGT)GATCCATTACAGATGGTTGTGGTTGCTGGGA NGG 10 8 17 8 24 0 100 5 10
    240605171319_z7za TCAAATCCCAGCAACCACAACCATCTGTAATG(+CAGTGT)GATCCAATGTCCTCTTCTGGTGTGTCTAAAG NGG 10 8 17 8 24 0 100 5 10
    240605171318_085g CATTGCATCATCTTTACATGAATGAGAAAAAG(+CAGTGT)GTACCTGTTGCACGTGGAAACCTTTAACCCA NGG 10 8 17 8 24 0 100 5 10