Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605171244_1gzw GACGGACATAATCCATGTTCTGTGTTCCAAAG(+CAGTGT)GATCCGAGGACAAATCCTAGGTCCCAACCCA NGG 10 8 17 8 24 0 100 5 10
    240605171243_pc6q GTCTAGCACTTACAGCATTAGCCTCCTTGATG(+CAGTGT)GATCCACTACTACCTGCATTTTGACTTCTCA NGG 10 8 17 8 24 0 100 5 10
    240605171242_b73x CTCTTTGAGAAGTCAAAATGCAGGTAGTAGTG(+CAGTGT)GATCCATCAAGGAGGCTAATGCTGTAAGTGC NGG 10 8 17 8 24 0 100 5 10
    240605171240_3eo3 TTGATGTGGGGGGGCCCAGCCCATTGTGGGTG(+CAGTGT)GTACCATCCTGGGCTGGTGGTCCTGGGTTCT NGG 10 8 17 8 24 0 100 5 10
    240605171239_1pou CTTATAGAACCCAGGACCACCAGCCCAGGATG(+CAGTGT)GTACCACCCACAATGGGCTGGGCCCCCCCAC NGG 10 8 17 8 24 0 100 5 10
    240605171237_mul5 AAGAAAGAAATGCATTAGCCTAAGTCCTTGTG(+CAGTGT)GATCCCAGGCTCCATTCTGCCATTCTCTTTC NGG 10 8 17 8 24 0 100 5 10
    240605171235_em4r AGTAGGAAAGAGAATGGCAGAATGGAGCCTGG(+CAGTGT)GATCCACAAGGACTTAGGCTAATGCATTTCT NGG 10 8 17 8 24 0 100 5 10
    240605171233_nhrh ACAAGCAACCGAGTAACAATCTTTCAACACTG(+CAGTGT)GTACCATGAGCAAATCCAGTGTTTTCTTCCA NGG 10 8 17 8 24 0 100 5 10
    240605171232_bdd0 GCAAATGGAAGAAAACACTGGATTTGCTCATG(+CAGTGT)GTACCAGTGTTGAAAGATTGTTACTCGGTTG NGG 10 8 17 8 24 0 100 5 10
    240605171229_3bjt CACTGCCTCTCAGTAGTTTTAAAATATCCCAG(+CAGTGT)GTACCACGTGCTTACTTTTATTACTTGGGTA NGG 10 8 17 8 24 0 100 5 10