Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605171212_w2mz TGTGAAGTTGTAGCGCAACTTATTTGACCACG(+CAGTGT)GTACCCTTAGAGAGGGGTGATGAACAGGCAT NGG 10 8 17 8 24 0 100 5 10
    240605171211_7vny GGTTTTCTAATGAAAACAAAAATGGATTTCAG(+CAGTGT)GTACCATGCTGATATTTGTTTTGTCTGTTTT NGG 10 8 17 8 24 0 100 5 10
    240605171209_rcfs CGTCTAAAACAGACAAAACAAATATCAGCATG(+CAGTGT)GTACCTGAAATCCATTTTTGTTTTCATTAGA NGG 10 8 17 8 24 0 100 5 10
    240605171208_nsg8 GTCCGTTGGTAAAACCTGTTTACAGTTGGCAG(+CAGTGT)GATCCTCTCATGAAGCTTACTATTGAGGCAA NGG 10 8 17 8 24 0 100 5 10
    240605171207_kug2 TGACTTTGCCTCAATAGTAAGCTTCATGAGAG(+CAGTGT)GATCCTGCCAACTGTAAACAGGTTTTACCAA NGG 10 8 17 8 24 0 100 5 10
    240605171205_tar3 TCCCACAAACATCTGTCTTGCCAGCTGGGCAG(+CAGTGT)GATCCAATTCCCCATACTGGCTTGTATACCC NGG 10 8 17 8 24 0 100 5 10
    240605171204_rddj TTTGAGGGTATACAAGCCAGTATGGGGAATTG(+CAGTGT)GATCCTGCCCAGCTGGCAAGACAGATGTTTG NGG 10 8 17 8 24 0 100 5 10
    240605171202_3nsd GTAAATGTATTTTTCCCTTTTAACTGTTCTTG(+CAGTGT)GATCCAAAGCATATGACCACTGCTCATTGAC NGG 10 8 17 8 24 0 100 5 10
    240605171201_h8zg TCAGTGTCAATGAGCAGTGGTCATATGCTTTG(+CAGTGT)GATCCAAGAACAGTTAAAAGGGAAAAATACA NGG 10 8 17 8 24 0 100 5 10
    240605171200_845p ATGGGAAAGTCAGGGGAGAACTGGGGCTAGAG(+CAGTGT)GTACCCTGTAAACCAGTTTCTAGATAAAAAC NGG 10 8 17 8 24 0 100 5 10