Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605171128_5cuy TTTCAATTAGCTTGAAGAAGTCTGTGGTGTTG(+CAGTGT)GTACCAGATGCTTCTCTAGTCCCCAGTTCCT NGG 10 8 17 8 24 0 100 5 10
    240605171127_51y6 CTTGACCGATTAGCTCGCATTTTATCTGGCTG(+CAGTGT)GTACCCTTTTCCCTCATAATGAGCTGCTTCT NGG 10 8 17 8 24 0 100 5 10
    240605171125_w1sn GAGGAAGAAGCAGCTCATTATGAGGGAAAAGG(+CAGTGT)GTACCAGCCAGATAAAATGCGAGCTAATCGG NGG 10 8 17 8 24 0 100 5 10
    240605171124_wf4d AAAAGGATATACCTTCTTCTCAGCACCTCATG(+CAGTGT)GTACCTTCTCCAAAATTGACCACATAATAGG NGG 10 8 17 8 24 0 100 5 10
    240605171122_fmlt TGTGACCTATTATGTGGTCAATTTTGGAGAAG(+CAGTGT)GTACCATGAGGTGCTGAGAAGAAGGTATATC NGG 10 8 17 8 24 0 100 5 10
    240605171121_hc5l ACGAAATGGACAAATTTCTGGACAGATACCAG(+CAGTGT)GTACCAAAGTTGAATCAGGATCAAGTTGACC NGG 10 8 17 8 24 0 100 5 10
    240605171119_kfg2 TAGAAGGTCAACTTGATCCTGATTCAACTTTG(+CAGTGT)GTACCTGGTATCTGTCCAGAAATTTGTCCAT NGG 10 8 17 8 24 0 100 5 10
    240605171118_nfij ATCTGTGTAAAGTTTGCATTAGTTAGAAGATG(+CAGTGT)GTACCGAGTTTAATTGACATAATAAAAGCTT NGG 10 8 17 8 24 0 100 5 10
    240605171117_nvir TGGGCAAGCTTTTATTATGTCAATTAAACTCG(+CAGTGT)GTACCATCTTCTAACTAATGCAAACTTTACA NGG 10 8 17 8 24 0 100 5 10
    240605171115_frxx GGTGTCAGCGTTTGGATGCTGATTATGGGGTG(+CAGTGT)GATCCCTGGATAAGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10