Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605171144_qfs1 ACTAGATTATGTTATATTTAATTAAATGTCAG(+CAGTGT)GATCCAGTAAGCCTGCAGTAAACTACATTTG NGG 10 8 17 8 24 0 100 5 10
    240605171142_kivx ATAACATGGCAATAATGAAGTGCTGTTTCCTG(+CAGTGT)GTACCTTGGGAATACTAAAGAAAGAAAAAGC NGG 10 8 17 8 24 0 100 5 10
    240605171139_rd0i GCAATGCTTTTTCTTTCTTTAGTATTCCCAAG(+CAGTGT)GTACCAGGAAACAGCACTTCATTATTGCCAT NGG 10 8 17 8 24 0 100 5 10
    240605171138_1zqu TTAAAAGAGAAACCAGAAACATCTAATCTGAG(+CAGTGT)GATCCAGACAATAAATATTACATGGATCATT NGG 10 8 17 8 24 0 100 5 10
    240605171137_5q96 CTCGGAATGATCCATGTAATATTTATTGTCTG(+CAGTGT)GATCCTCAGATTAGATGTTTCTGGTTTCTCT NGG 10 8 17 8 24 0 100 5 10
    240605171135_004d TTAGATTATGTATGAAAATTCATACAATACTG(+CAGTGT)GATCCTATGACTAAAATGAAAATAGTAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605171134_yayd TGCTGCTCCTACTATTTTCATTTTAGTCATAG(+CAGTGT)GATCCAGTATTGTATGAATTTTCATACATAA NGG 10 8 17 8 24 0 100 5 10
    240605171133_o5jy GTCCCTGGTTCTGCTCAAACAGTTACTTCAAG(+CAGTGT)GTACCCTTAAAAGGGCTCCATTAAACAAGAA NGG 10 8 17 8 24 0 100 5 10
    240605171131_6bzg GTACATTCTTGTTTAATGGAGCCCTTTTAAGG(+CAGTGT)GTACCTTGAAGTAACTGTTTGAGCAGAACCA NGG 10 8 17 8 24 0 100 5 10
    240605171130_u8ah TAGGCAGGAACTGGGGACTAGAGAAGCATCTG(+CAGTGT)GTACCAACACCACAGACTTCTTCAAGCTAAT NGG 10 8 17 8 24 0 100 5 10