Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605171158_zzvw GAGAAGTTTTTATCTAGAAACTGGTTTACAGG(+CAGTGT)GTACCTCTAGCCCCAGTTCTCCCCTGACTTT NGG 10 8 17 8 24 0 100 5 10
    240605171157_g8ln AAAGTAAGCAAATTGTAGCATAAGATATATTG(+CAGTGT)GATCCACTCAGGAAGGTTGTATTACATTAGG NGG 10 8 17 8 24 0 100 5 10
    240605171156_zpbg TATTTCCTAATGTAATACAACCTTCCTGAGTG(+CAGTGT)GATCCAATATATCTTATGCTACAATTTGCTT NGG 10 8 17 8 24 0 100 5 10
    240605171154_63zx GGAACAAAGCACCATTGGCCTCTAGCTCTGGG(+CAGTGT)GATCCTACAGCGTCACCTTCCTCCTAGACAG NGG 10 8 17 8 24 0 100 5 10
    240605171152_vhnc TCCTGCTGTCTAGGAGGAAGGTGACGCTGTAG(+CAGTGT)GATCCCCAGAGCTAGAGGCCAATGGTGCTTT NGG 10 8 17 8 24 0 100 5 10
    240605171151_n745 AAGGTTTGCCCTTTAGTTTAGAAACCAGATAG(+CAGTGT)GATCCCATCCTTGAATTTTAAATGAAGTCAT NGG 10 8 17 8 24 0 100 5 10
    240605171149_xuzt TGCAAATGACTTCATTTAAAATTCAAGGATGG(+CAGTGT)GATCCTATCTGGTTTCTAAACTAAAGGGCAA NGG 10 8 17 8 24 0 100 5 10
    240605171148_l7ry GCAGTGCTGGTAGAATCCAGTAGAGGATATTG(+CAGTGT)GATCCTCTGTGACTAGAATTACAGATAGTTA NGG 10 8 17 8 24 0 100 5 10
    240605171147_vphw GTTCATAACTATCTGTAATTCTAGTCACAGAG(+CAGTGT)GATCCAATATCCTCTACTGGATTCTACCAGC NGG 10 8 17 8 24 0 100 5 10
    240605171145_q4v9 CCAGACAAATGTAGTTTACTGCAGGCTTACTG(+CAGTGT)GATCCTGACATTTAATTAAATATAACATAAT NGG 10 8 17 8 24 0 100 5 10