Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605171054_7p3a GGTGTCAGCGTTTGGAAGCTGATTATGGGATG(+CAGTGT)GATCCCTGGATATGGCAATCACTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605171052_3i1o TGAGCTCTTAGCCCTAGGTTTTAAGAGCCTCG(+CAGTGT)GATCCCAACTATGTTGAATTTCCTAAACTAC NGG 10 8 17 8 24 0 100 5 10
    240605171051_4scp ACATCGTAGTTTAGGAAATTCAACATAGTTGG(+CAGTGT)GATCCGAGGCTCTTAAAACCTAGGGCTAAGA NGG 10 8 17 8 24 0 100 5 10
    240605171049_fd82 TCCTCAGAAAATTGGACATAGTACTACCGGAG(+CAGTGT)GATCCAGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240605171048_99ri TTCTGGATATATGCCCAGGAGAGGTATTGCTG(+CAGTGT)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605171045_16n2 GGGTGGACCATGTAGAGACTGCCTTATCCAGG(+CAGTGT)GATCCACCCCATAATCAGCATCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240605171043_ewx0 GGTGTCAGCGTTTGGATGCTGATTATGGGGTG(+CAGTGT)GATCCCTGGATAAGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605171042_vfz5 TCCCAAAGTTATTTAAACATCAGTAAGACAAG(+CAGTGT)GTACCACTTCATACAGTGAAAAGTGGAATCT NGG 10 8 17 8 24 0 100 5 10
    240605171040_uq2j TCTGTAGATTCCACTTTTCACTGTATGAAGTG(+CAGTGT)GTACCTTGTCTTACTGATGTTTAAATAACTT NGG 10 8 17 8 24 0 100 5 10
    240605171039_ew2y TCACTTCAATCTCTTCGTCACATATTGGGGTG(+CAGTGT)GTACCAAGGAAGGTGACCAGTATTGTTTACA NGG 10 8 17 8 24 0 100 5 10