Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605171023_jqsi CTGGGTAAGGAAACAAGCTGCCTCAAGTGTGG(+CAGTGT)GTACCATGAGGTCCAACAGGACAGAACAGAC NGG 10 8 17 8 24 0 100 5 10
    240605171021_1nit TTGTTAAAGGATTTGAGGGTTTTTCTCTGCAG(+CAGTGT)GTACCACCTGTAAATAAGAAGAAAGTAGGGG NGG 10 8 17 8 24 0 100 5 10
    240605171019_5068 TTTGGCCCCTACTTTCTTCTTATTTACAGGTG(+CAGTGT)GTACCTGCAGAGAAAAACCCTCAAATCCTTT NGG 10 8 17 8 24 0 100 5 10
    240605171018_6max ATTTCTTCAAAGAGAGACAAGGTTCATGCCTG(+CAGTGT)GTACCATTTTTTTTCTAATGAAATTAACTTT NGG 10 8 17 8 24 0 100 5 10
    240605171016_sp8v CTAAAAAAGTTAATTTCATTAGAAAAAAAATG(+CAGTGT)GTACCAGGCATGAACCTTGTCTCTCTTTGAA NGG 10 8 17 8 24 0 100 5 10
    240605171015_526o TACCTTTAATCTTAGCACTTGAGAGGCAGAGG(+CAGTGT)GATCCCCGAGTTAGAGATCAGCCTTGTTTAT NGG 10 8 17 8 24 0 100 5 10
    240605171012_6gg9 TTGAAATAAACAAGGCTGATCTCTAACTCGGG(+CAGTGT)GATCCCTCTGCCTCTCAAGTGCTAAGATTAA NGG 10 8 17 8 24 0 100 5 10
    240605171010_s3lk ATTTCTTCAAAGAGAGACAAGGTTCATGCCTG(+CAGTGT)GTACCATTTTTTTTCTAATGAAATTAACTTT NGG 10 8 17 8 24 0 100 5 10
    240605171009_zmmz CTAAAAAAGTTAATTTCATTAGAAAAAAAATG(+CAGTGT)GTACCAGGCATGAACCTTGTCTCTCTTTGAA NGG 10 8 17 8 24 0 100 5 10
    240605171008_l4zj GTGGTGCACTCCAACAGCATTTTAAGTACTTG(+CAGTGT)GATCCCACGTTGATCTTATAAACACAAGCAA NGG 10 8 17 8 24 0 100 5 10