Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605171006_fnva TTTTATTGCTTGTGTTTATAAGATCAACGTGG(+CAGTGT)GATCCAAGTACTTAAAATGCTGTTGGAGTGC NGG 10 8 17 8 24 0 100 5 10
    240605171004_n9j0 TCTCTGTCTGACTTCCTATGCATGGGGGCTGG(+CAGTGT)GATCCATTTCTAAGGAGAATGGATAAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605171003_joat GCCTACTTCCTTATCCATTCTCCTTAGAAATG(+CAGTGT)GATCCCAGCCCCCATGCATAGGAAGTCAGAC NGG 10 8 17 8 24 0 100 5 10
    240605171002_dkyn GTGGTGCACTCCAACAGCATTTTAAGTACTTG(+CAGTGT)GATCCCACGTTGATCTTATAAACACAAGCAA NGG 10 8 17 8 24 0 100 5 10
    240605171000_9wo5 TTTTATTGCTTGTGTTTATAAGATCAACGTGG(+CAGTGT)GATCCAAGTACTTAAAATGCTGTTGGAGTGC NGG 10 8 17 8 24 0 100 5 10
    240605170958_ovua TCTCTGTCTGACTTCCTATGCATGGGGGCTGG(+CAGTGT)GATCCATTTCTAAGGAGAATGGATAAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605170957_azrk GCCTACTTCCTTATCCATTCTCCTTAGAAATG(+CAGTGT)GATCCCAGCCCCCATGCATAGGAAGTCAGAC NGG 10 8 17 8 24 0 100 5 10
    240605170956_0fhi ACAAAGTCCTTTAATTTAAAAAAAAAAAAAAG(+CAGTGT)GTACCTGATTCTCACTTCTCTCAATCTTCTA NGG 10 8 17 8 24 0 100 5 10
    240605170955_321x GTAAATAGAAGATTGAGAGAAGTGAGAATCAG(+CAGTGT)GTACCTTTTTTTTTTTTTTAAATTAAAGGAC NGG 10 8 17 8 24 0 100 5 10
    240605170952_9x15 GTTACAGATTGCTCTTTATTATAATACCAGTG(+CAGTGT)GTACCCAAAGAAAAAAGCAGAGCATTATGTA NGG 10 8 17 8 24 0 100 5 10