Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605170904_jdf6 TGTATCTCAGTAAAACGCTCTGTTGACTATGG(+CAGTGT)GTACCTCATTTCTCCTCTGAATTTTTCATGT NGG 10 8 17 8 24 0 100 5 10
    240605170902_gmkw TGGTACTAGGGTTTCCTGTAAACACCTTGTGG(+CAGTGT)GTACCTCATAAGCAGCTGAGATTATTAGTGT NGG 10 8 17 8 24 0 100 5 10
    240605170901_lrt2 ACCAAACACTAATAATCTCAGCTGCTTATGAG(+CAGTGT)GTACCCACAAGGTGTTTACAGGAAACCCTAG NGG 10 8 17 8 24 0 100 5 10
    240605170859_0rbd TGAAAATACTTGAAGTATTTCCTGACCTCCAG(+CAGTGT)GTACCACTTTAAGCATGCTAGATATCTAGGT NGG 10 8 17 8 24 0 100 5 10
    240605170857_kgtq TCCCAACCTAGATATCTAGCATGCTTAAAGTG(+CAGTGT)GTACCTGGAGGTCAGGAAATACTTCAAGTAT NGG 10 8 17 8 24 0 100 5 10
    240605170856_svar CTCTAGTGCTTTATAGCAAAAGCTATAAAGTG(+CAGTGT)GTACCCGGGTGACCAGCATTGCCATGGTTAC NGG 10 8 17 8 24 0 100 5 10
    240605170853_eq2s TGGATGTAACCATGGCAATGCTGGTCACCCGG(+CAGTGT)GTACCACTTTATAGCTTTTGCTATAAAGCAC NGG 10 8 17 8 24 0 100 5 10
    240605170851_bof6 CCACGCTGCTTCAAGCTAACTGCACCAGATTG(+CAGTGT)GATCCAGCCATCAGCACTCCCAGAGACTCCT NGG 10 8 17 8 24 0 100 5 10
    240605170850_0nwt TTTTAAGGAGTCTCTGGGAGTGCTGATGGCTG(+CAGTGT)GATCCAATCTGGTGCAGTTAGCTTGAAGCAG NGG 10 8 17 8 24 0 100 5 10
    240605170848_q7th TCACAAGAACATGATCTGTCCCGGCAGTGAAG(+CAGTGT)GTACCAAGAGCAGCTTGGCCCATGGTGGCGA NGG 10 8 17 8 24 0 100 5 10