Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605170830_8jxv TTGAAATATTTCTGTCAGGTTCCACTGGCTTG(+CAGTGT)GTACCTTGTATCCCATAGGACATTTAAAAGG NGG 10 8 17 8 24 0 100 5 10
    240605170829_49r1 TGCAGGAGGTTTGTCGCTGAAGCCATGCGATG(+CAGTGT)GATCCAGAGAGACTTTTTAATAATCCTTAGG NGG 10 8 17 8 24 0 100 5 10
    240605170828_19fe TATTACCTAAGGATTATTAAAAAGTCTCTCTG(+CAGTGT)GATCCATCGCATGGCTTCAGCGACAAACCTC NGG 10 8 17 8 24 0 100 5 10
    240605170825_1dhr TCCTGTGGTTTTGTAAACAGAGATATTGGGGG(+CAGTGT)GATCCTCCTTGAAATAGTTTGGAAGAATTGC NGG 10 8 17 8 24 0 100 5 10
    240605170823_j6q5 ACAGTGCAATTCTTCCAAACTATTTCAAGGAG(+CAGTGT)GATCCCCCCAATATCTCTGTTTACAAAACCA NGG 10 8 17 8 24 0 100 5 10
    240605170821_376i AGAACTATACATAGTGAGACCATGTAACCCTG(+CAGTGT)GATCCTAACAACACAGTCTGTAAATAAAAGT NGG 10 8 17 8 24 0 100 5 10
    240605170819_ytjs AATAAACTTTTATTTACAGACTGTGTTGTTAG(+CAGTGT)GATCCAGGGTTACATGGTCTCACTATGTATA NGG 10 8 17 8 24 0 100 5 10
    240605170818_u5cq TAGGTGACATTCAGAGATGCTGTGTTGGGCTG(+CAGTGT)GTACCAGAATGCATTGATGTGTGTGCTTTAC NGG 10 8 17 8 24 0 100 5 10
    240605170816_i1nh CAACGGTAAAGCACACACATCAATGCATTCTG(+CAGTGT)GTACCAGCCCAACACAGCATCTCTGAATGTC NGG 10 8 17 8 24 0 100 5 10
    240605170815_vu3h TATGCTACTTGCACTGTGACATGGCATTTGAG(+CAGTGT)GATCCCTGACTTACAGACAAAGCTTAAAATG NGG 10 8 17 8 24 0 100 5 10