Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605170847_fwfq CCCATTCGCCACCATGGGCCAAGCTGCTCTTG(+CAGTGT)GTACCTTCACTGCCGGGACAGATCATGTTCT NGG 10 8 17 8 24 0 100 5 10
    240605170845_3dal CCTACAGTGACTTGATGTACCAGGGTGGGTTG(+CAGTGT)GTACCCAGAGGAGCCTCCATCTTCTCAGAGG NGG 10 8 17 8 24 0 100 5 10
    240605170844_u153 CTTCACCTCTGAGAAGATGGAGGCTCCTCTGG(+CAGTGT)GTACCAACCCACCCTGGTACATCAAGTCACT NGG 10 8 17 8 24 0 100 5 10
    240605170841_z948 TTGTAAAAGGCCTACCTTCAAGACCACCCATG(+CAGTGT)GATCCTCAAACCTATTAATCTGTTAAGGACG NGG 10 8 17 8 24 0 100 5 10
    240605170840_ra8t GGCTCCGTCCTTAACAGATTAATAGGTTTGAG(+CAGTGT)GATCCATGGGTGGTCTTGAAGGTAGGCCTTT NGG 10 8 17 8 24 0 100 5 10
    240605170838_cvzm GTCACATTGTATCCAGGCAGGAAGCAGAGATG(+CAGTGT)GATCCAGGTGCTCGGTCCCCTCTCCTGTTCT NGG 10 8 17 8 24 0 100 5 10
    240605170836_fk7s CTATAAGAACAGGAGAGGGGACCGAGCACCTG(+CAGTGT)GATCCATCTCTGCTTCCTGCCTGGATACAAT NGG 10 8 17 8 24 0 100 5 10
    240605170835_gmea GTAAACACACAAACAGTAAGCTTAGCTGTTGG(+CAGTGT)GATCCTGCCCCGAGGTCACCACTCCCTTCAT NGG 10 8 17 8 24 0 100 5 10
    240605170833_o9pq ATGGAATGAAGGGAGTGGTGACCTCGGGGCAG(+CAGTGT)GATCCCAACAGCTAAGCTTACTGTTTGTGTG NGG 10 8 17 8 24 0 100 5 10
    240605170832_vilm GTGGGCCTTTTAAATGTCCTATGGGATACAAG(+CAGTGT)GTACCAAGCCAGTGGAACCTGACAGAAATAT NGG 10 8 17 8 24 0 100 5 10