Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605170813_m3j3 TGTGTCATTTTAAGCTTTGTCTGTAAGTCAGG(+CAGTGT)GATCCTCAAATGCCATGTCACAGTGCAAGTA NGG 10 8 17 8 24 0 100 5 10
    240605170812_n747 AAAGCTCATCACAAGGTCTACTAAGCATTAAG(+CAGTGT)GATCCTCATTATAAGTCCTCTGTACCACTTT NGG 10 8 17 8 24 0 100 5 10
    240605170810_a815 AAAAAAAAGTGGTACAGAGGACTTATAATGAG(+CAGTGT)GATCCTTAATGCTTAGTAGACCTTGTGATGA NGG 10 8 17 8 24 0 100 5 10
    240605170809_iiqx AATACTGACTCTCATTAGATATGATCTTATAG(+CAGTGT)GATCCATACCCTCCCTAGGACCAAATATATC NGG 10 8 17 8 24 0 100 5 10
    240605170808_ob9h CATCAGATATATTTGGTCCTAGGGAGGGTATG(+CAGTGT)GATCCTATAAGATCATATCTAATGAGAGTCA NGG 10 8 17 8 24 0 100 5 10
    240605170805_0p2j GGCATGGCTCCTCGCCGCAGCTGTCTCCCTTG(+CAGTGT)GTACCGACCTCTCTGTGGGCTGGAGAGAGGC NGG 10 8 17 8 24 0 100 5 10
    240605170803_3q6x CTTAGGCCTCTCTCCAGCCCACAGAGAGGTCG(+CAGTGT)GTACCAAGGGAGACAGCTGCGGCGAGGAGCC NGG 10 8 17 8 24 0 100 5 10
    240605170802_wcov CCTTGTCAAACCAAGTTGTCATGGATGCTTTG(+CAGTGT)GTACCCCTCCCCTCAGGGGCTTTGGTATTAC NGG 10 8 17 8 24 0 100 5 10
    240605170800_s4gl TTCTTGTAATACCAAAGCCCCTGAGGGGAGGG(+CAGTGT)GTACCAAAGCATCCATGACAACTTGGTTTGA NGG 10 8 17 8 24 0 100 5 10
    240605170758_xyae GTGGGGTTCCTGCGCTGTGTGTGCCTACTCAG(+CAGTGT)GTACCCCAACATTGCTTTCCCTAGCTCTCTG NGG 10 8 17 8 24 0 100 5 10