Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605171403_db3t AGAGAGAACAGAGGCTGACAGACAGATCCACG(+CAGTGT)GTACCAAGCGGCTTTATTGGAAGCTCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240605171401_bx1u TGGAGGGCTTGGACATATCCTTTTTCTTGCAG(+CAGTGT)GTACCTTGAGAACTCAAGGATGTAACATCCA NGG 10 8 17 8 24 0 100 5 10
    240605171400_uwqn GTCCTTGGATGTTACATCCTTGAGTTCTCAAG(+CAGTGT)GTACCTGCAAGAAAAAGGATATGTCCAAGCC NGG 10 8 17 8 24 0 100 5 10
    240605171359_00x1 GTTGAGGAGAGCAGCATATTCATGGAGCGCTG(+CAGTGT)GATCCTCTGGGGCAGCCCCCAGCGGAGGTGA NGG 10 8 17 8 24 0 100 5 10
    240605171357_d37w CTGATTCACCTCCGCTGGGGGCTGCCCCAGAG(+CAGTGT)GATCCAGCGCTCCATGAATATGCTGCTCTCC NGG 10 8 17 8 24 0 100 5 10
    240605171356_hj2z CTAGACTGCAGTGCTTCCAATAAAGCTGCTTG(+CAGTGT)GTACCGTGGATCTGTCTGTCGGTTGTCTGTT NGG 10 8 17 8 24 0 100 5 10
    240605171354_uhfr GAGAGAACAGACAACCGACAGACAGATCCACG(+CAGTGT)GTACCAAGCAGCTTTATTGGAAGCACTGCAG NGG 10 8 17 8 24 0 100 5 10
    240605171352_1gn8 GTCATGTAACCCCTATGTCAGTGTCACCTTTG(+CAGTGT)GTACCCCACCTCTTTGAGTCAATTCTGCTCC NGG 10 8 17 8 24 0 100 5 10
    240605171350_ueux GAAAAGGAGCAGAATTGACTCAAAGAGGTGGG(+CAGTGT)GTACCAAAGGTGACACTGACATAGGGGTTAC NGG 10 8 17 8 24 0 100 5 10
    240605171348_i1co TTGATTCTTTCAGGATGTGACGCCCACTCTGG(+CAGTGT)GATCCAGTCCTGGTTGATGTCAGCATGCCAC NGG 10 8 17 8 24 0 100 5 10