Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605170426_brv0 TTATGATTGTTTGTTCCTGAACACTTCTGCAG(+CAGTGT)GTACCTATAACCTGAAAGTGTTTCTTTTTTA NGG 10 8 17 8 24 0 100 5 10
    240605170424_5z4l AGCAGCAGCTGTACACTTCTGGAATGACACAG(+CAGTGT)GTACCAACACAGAGTTCATGGCCCCAGGAAC NGG 10 8 17 8 24 0 100 5 10
    240605170423_zrb5 ATGTGGTTCCTGGGGCCATGAACTCTGTGTTG(+CAGTGT)GTACCTGTGTCATTCCAGAAGTGTACAGCTG NGG 10 8 17 8 24 0 100 5 10
    240605170421_1zkn ATAGGCCTGTCACTGCAAAACCACCGTCCCTG(+CAGTGT)GTACCAACTTTTGTCTTAGTTACGATTTTAT NGG 10 8 17 8 24 0 100 5 10
    240605170419_jb1c CAGCAATAAAATCGTAACTAAGACAAAAGTTG(+CAGTGT)GTACCAGGGACGGTGGTTTTGCAGTGACAGG NGG 10 8 17 8 24 0 100 5 10
    240605170417_m3qf ATGGGAGAGAGTGACCGAGCTAGGAACAGAGG(+CAGTGT)GATCCCAGCCACTCCAAGGTAGAGTCTGTGT NGG 10 8 17 8 24 0 100 5 10
    240605170416_hbha TCCATACACAGACTCTACCTTGGAGTGGCTGG(+CAGTGT)GATCCCTCTGTTCCTAGCTCGGTCACTCTCT NGG 10 8 17 8 24 0 100 5 10
    240605170414_ifbc CCTGGTGCCCTTGGAGGCAGTAAGAGGGCATG(+CAGTGT)GATCCCCTGGAACTGGAGTTACAGACAGTTG NGG 10 8 17 8 24 0 100 5 10
    240605170412_2vpp GTTCACAACTGTCTGTAACTCCAGTTCCAGGG(+CAGTGT)GATCCATGCCCTCTTACTGCCTCCAAGGGCA NGG 10 8 17 8 24 0 100 5 10
    240605170410_tjjh CTATCTTCAGACACAACCAGAGGAGGGCATTG(+CAGTGT)GATCCTATTACCAATGGTTGTAAGCTACCAT NGG 10 8 17 8 24 0 100 5 10